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Chinese Optics Letters

Chinese Optics Letters


  • Editor: Zhizhan Xu
  • Vol. 10, Iss. 5 — May. 1, 2012
  • pp: 051901–

Investigation of transient two-photon excited fluorescence in biological tissue

Yishen Qiu, Enguo Chen, Gaoming Li, and Hui Li  »View Author Affiliations

Chinese Optics Letters, Vol. 10, Issue 5, pp. 051901- (2012)

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The fluorescence power from biological tissue excited by a femtosecond laser pulse compared with excitation power does not appear to obey a simple quadratic relationship given by the steady non-linear theory. A more reliable analysis is developed based on transient two-photon absorption because the response time of two-photon absorption is longer than the width of a femtosecond pulse. Good agreement is obtained between the theoretical analysis and the experimental results of fluorescence power versus excitation power. This letter offers potential value to non-linear optics in biological tissues.

© 2012 Chinese Optics Letters

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(190.4180) Nonlinear optics : Multiphoton processes
(260.2510) Physical optics : Fluorescence

ToC Category:
Nonlinear Optics

Yishen Qiu, Enguo Chen, Gaoming Li, and Hui Li, "Investigation of transient two-photon excited fluorescence in biological tissue," Chin. Opt. Lett. 10, 051901- (2012)

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  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  2. C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).
  3. P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).
  4. V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).
  5. F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).
  6. W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).
  7. J. B. Dominguez and Y. Berube-Lauzire, Biomed. Opt. Express 2, 817 (2011).
  8. R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).
  9. H. Quan and Z. Guo, Opt. Express 12, 449 (2004).
  10. Y. R. Shen, The Principles of Nonlinear Optics (John Wiley & Sons, New York, 1984).
  11. Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).
  12. R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).
  13. J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

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