Recent advances in two-photon imaging: technology developments and biomedical applications
Chinese Optics Letters, Vol. 11, Issue 1, pp. 011703- (2013)
Acrobat PDF (1247 KB)
Abstract
In the past two decades, two-photon microscopy (TPM) transforms biomedical research, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technology including novel laser sources, new image acquisition paradigms, and microendoscopic imaging systems. Then, we survey the capabilities of TPM imaging of biological relevant molecules such as nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
© 2013 Chinese Optics Letters
OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.0180) Microscopy : Microscopy
(190.0190) Nonlinear optics : Nonlinear optics
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy
Citation
Yu Chen, Hengchang Guo, Wei Gong, Luye Qin, Hossein Aleyasin, Rajiv R Ratan, Sunghee Cho, Jianxin Chen, and Shusen Xie, "Recent advances in two-photon imaging: technology developments and biomedical
applications," Chin. Opt. Lett. 11, 011703- (2013)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-11-1-011703
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy (OL)
- A simple scanless two-photon fluorescence microscope using selective plane illumination (OE)
- Fast image analysis in polarization SHG microscopy. (OE)
- Influence of laser parameters and staining on femtosecond laser-based intracellular nanosurgery (BOE)
- Investigation on activating individual living monocytic U937 cell by interleukin-6 using Raman tweezers (COL)
Related Conference Papers
- Flexible Scanning MicroEndoscope for Two-Photon Fluorescence and SHG Imaging
- Multiphoton Imaging of an in vitro Ovarian Tissue Model
- Optical Second Order Nonlinearity in Collagen: Molecular Origins Determined by Sum-Frequency, Infrared and Raman Spectroscopy
- Stroking the Synapse: Insight into Ischemic Damage and Recovery from <i>in vivo</i> 2-Photon Imaging of Individual Synapses
- Two-Photon Luminescence Imaging Using Gold Nanorods as Bright Contrast Agents
« Previous Article | Next Article »
OSA is a member of 