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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Editor: Zhizhan Xu
  • Vol. 11, Iss. 9 — Sep. 1, 2013
  • pp: 091702–

Measurement of the binding force between RAS protein and a pathologic BRAF mutant using optical tweezers

Cheng Wen and Anpei Ye  »View Author Affiliations


Chinese Optics Letters, Vol. 11, Issue 9, pp. 091702- (2013)


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Abstract

Activating mutants in rat sarcoma (RAS) and B-rapid accelerated fibrosarcoma (BRAF) are found in at least a third of cases of human tumors and melanoma; hence, numerous therapeutic treatments target this pathway. In this letter, we study the adhesion force of RAS-coated beads with BRAF-coated beads, BRAF (A246P) mutant-coated beads, and GST-coated beads using optical tweezers. One full and two fractional RAS-BRAF specific binding modes are identified using the rupture force distribution. The koff (0) of the full binding mode in RAS-BRAF is 3.71×10-4/s and 1.16×10-4s-1 in RAS-BRAF (A246P), whereas the xb is around 3 \times 10-10 m in both groups.

© 2013 Chinese Optics Letters

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology

ToC Category:
Medical Optics and Biotechnology

Citation
Cheng Wen and Anpei Ye, "Measurement of the binding force between RAS protein and a pathologic BRAF mutant using optical tweezers," Chin. Opt. Lett. 11, 091702- (2013)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-11-9-091702

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