Characteristics of blood fluorescence spectra using low-level, 457.9-nm excitation from Ar+ laser
Chinese Optics Letters, Vol. 2, Issue 3, pp. 160-161 (2004)
Acrobat PDF (386 KB)
Abstract
We measured the fluorescence spectra of the whole blood, the red blood cell (RBC) and the hemoglobin using 457.9-nm Ar+ laser excitation. It was found that the fluorescence spectra of the whole blood and the RBC have much similarities in the intensity, the emission peaks and the emitting region, and abundant peaks can be found. But for the hemoglobin, fluorescence could only be found in the wavelength range 580-650 nm. It was concluded that in the wavelength range of 650-850 nm, the fluorescence spectra were emitted by the new fluorophores generated by the breakdown of some weak bonds on the RBC membrane, such as the C-C bond and the C-N bond. In the wavelength range of 590-650 nm, the fluorescence spectra are mainly emitted by the hemoglobin, but the hemoglobin solution of cracked RBC has a strong quencher effect on the fluorescence spectrum. The experimental result and the theoretical analysis are meaningful for the medical diagnostics and the therapy.
© 2005 Chinese Optics Letters
OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(270.0270) Quantum optics : Quantum optics
(300.0300) Spectroscopy : Spectroscopy
Citation
Shumei Gao, Xiufeng Lan, Ying Liu, Zhonghua Shen, Jian Lu, and Xiaowu Ni, "Characteristics of blood fluorescence spectra using low-level, 457.9-nm excitation from Ar+ laser," Chin. Opt. Lett. 2, 160-161 (2004)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-2-3-160
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Interferometric measurement of tear film thickness by use of spectral oscillations (JOSAA)
- Skin lesion classification using oblique-incidence diffuse reflectance spectroscopic imaging (AO)
- High-sensitivity and specificity of laser-induced autofluorescence spectra for detection of colorectal cancer with an artificial neural network (AO)
- Determination of optimal source-detector separation in measuring chromophores in layered tissue with diffuse reflectance (COL)
- Microfluidic devices for terahertz spectroscopy of biomolecules (OE)
Related Conference Papers
- Topological decomposition of composite quantum state spaces
- Topological decomposition of composite quantum state spaces
- Quantum information with atomic ensembles
- Quantum information with atomic ensembles
- Atom chips for quantum information research
- Atom chips for quantum information research
- Free space Quantum Cryptography at 1.9km range
- Free space Quantum Cryptography at 1.9km range
- Experimental six-state quantum cryptography
- Experimental six-state quantum cryptography
« Previous Article | Next Article »
OSA is a member of 