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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 2, Iss. 3 — Mar. 10, 2004
  • pp: 162–164

Cross-talk correction in dual-labeled fluorescent microarray scanning

Liqiang Wang, Zukang Lu, and Xuxiang Ni  »View Author Affiliations


Chinese Optics Letters, Vol. 2, Issue 3, pp. 162-164 (2004)


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Abstract

Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.

© 2005 Chinese Optics Letters

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.2520) Microscopy : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence

Citation
Liqiang Wang, Zukang Lu, and Xuxiang Ni, "Cross-talk correction in dual-labeled fluorescent microarray scanning," Chin. Opt. Lett. 2, 162-164 (2004)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-2-3-162


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