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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 3, Iss. S1 — Aug. 28, 2005
  • pp: S143–S146

Confocal microscopy of FM 4-64 tagged membranes in the living fungus Trichoderma reesei

Hong Yu, Debra Birch, Junior Te'o, Ewa Goldys, and Helena Nevalainen  »View Author Affiliations


Chinese Optics Letters, Vol. 3, Issue S1, pp. S143-S146 (2005)


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Abstract

Visualization of the protein secretory pathway in the high secreting trichoderma reesei (T. reesei) hyphae using confocal microscopy will lead to better understanding of the cellular mechanisms involved in protein secretion and contribute to the identification of bottlenecks in the secretion of foreign proteins from fungi. An introduction into visualization approaches involved the application of the fluorescent dye FM 4-64 for staining of membrane-based structures in T. reesei hyphae. Confocal microscopy studies were carried out with 24-hour old cultures of the T. reesei strain Rut-C30. Staining of the fungal hyphae and characteristics of FM 4-64 including spectral properties, time course of the labeling and double labeling with other specific organelle dyes will be discussed.

© 2005 Chinese Optics Letters

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy

Citation
Hong Yu, Debra Birch, Junior Te'o, Ewa Goldys, and Helena Nevalainen, "Confocal microscopy of FM 4-64 tagged membranes in the living fungus Trichoderma reesei," Chin. Opt. Lett. 3, S143-S146 (2005)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-3-S1-S143


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