High signal-to-noise surface enhanced Raman scattering (SERS) signal of rat serum was obtained in this article. Two methods were presented to enhance the intensity of rat serum SERS signal. The novel colloid silver was synthesized using heating method by microwave and it was introduced to be the active site of SERS, "hot sites" could be detected in the active site. At same time the high numerical aperture (NA) oil immersed object lens was applied in micro-Raman system so that some new peaks come up in SERS spectrum of rat serum because of the evanescent wave was employed to excite the sample.
© 2005 Chinese Optics Letters
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.5860) Scattering : Scattering, Raman
(290.5910) Scattering : Scattering, stimulated Raman
(300.6450) Spectroscopy : Spectroscopy, Raman
Kun Liu, Shifa Wu, Yi Zhang, and Hui Liu, "Application of surface enhanced Raman scattering spectroscopy in rat serum," Chin. Opt. Lett. 3, S161-S163 (2005)