OSA's Digital Library

Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 3, Iss. S1 — Aug. 28, 2005
  • pp: S235–S236

Effects of alcohol on fluorescence spectra of erythrocyte solution

Xiufeng Lan, Ying Liu, Xiaosen Luo, Zhonghua Shen, Jian Lu, and Xiaowu Ni  »View Author Affiliations


Chinese Optics Letters, Vol. 3, Issue S1, pp. S235-S236 (2005)


View Full Text Article

Acrobat PDF (261 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Based on the experimental researches on the fluorescence spectra of 1% erythrocyte solution with various concentration of alcohol excited by violet light-emitting diode LED light at 407 nm, the mechanism of alcohol on the fluorescence spectra of erythrocyte solution is investigated theoretically. The experiment results indicate that induced by the LED light at 407 nm, erythrocyte solution with the concentration of 1% can emitting striking spectra with two fluorescence regions. One is the short-wave fluorescence region from 430 to 525 nm, and the other is the long-wave fluorescence region from 580 to 750 nm. When the concentration of alcohol in erythrocyte solution increasing, the fluorescence intensity of short-wave area decrease while the fluorescence intensity of long-wave area increases. Combining the blood absorb spectra to the experiment results, it is shown that the formation of the short-wave fluorescence area is because the solution transmits the fluorescence spectra and the self-absorption of erythrocyte. The long-wave fluorescence region comes from porphyrin such as protoporphyrin, zinc porphyrin etc.. And there is resonance energy transmission between the short-wave fluorophores and the long-wave ones. According to the experiment results and the physical theory in erythrocyte fluorescence, it is found that alcohol make higher self-absorption ratio of the erythrocyte which improves the resonance energy transmission between fluorophores in the two wave bands. The result will offer experimental and theoretical reference for examining the alcohol content in blood.

© 2005 Chinese Optics Letters

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

Citation
Xiufeng Lan, Ying Liu, Xiaosen Luo, Zhonghua Shen, Jian Lu, and Xiaowu Ni, "Effects of alcohol on fluorescence spectra of erythrocyte solution," Chin. Opt. Lett. 3, S235-S236 (2005)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-3-S1-S235


Sort:  Year  |  Journal  |  Reset

References

  1. S. M. de la Monte and J. R. Wands, Cellular and Molecular Life Sciences 59, 882 (2002).
  2. Z. Gdovinova, Comparative Clinical Pathology 11, 77 (2002).
  3. M. Hillbom and S. Juvella, National Institutes of Health, Bethesda, 1996, 63.
  4. K. Karthikeyan, V. Masilamani, and S. Govindasamy, Pathology Oncology Research 5, 46 (1999).
  5. J.-L. Boulnios, Lasers Med. Sci. 1, 47 (1986).
  6. Z. L. Chen, Z. R. Duan, and H. F. Nie, Chin. J. Lasers (in Chinese) 21, 77 (1994).
  7. D. Kessel, T. J. Dougherty, Porphyrin Phorosensitization (Plenum Press, New York, 1983).
  8. Perkin-Elmer Ltd., An Intrduction to Fluorescence in Biological Analysis (2000).
  9. S. M. Gao, X. S. Luo, X. F. Lan, Y. Liu, J. Lu, and X. W. Ni, Chin. J. Lasers B 11, 315 (2002).
  10. S. Gao, X. Lan, Y. Liu, Z. Shen, J. Lu, and X. Ni, Chin. Opt. Lett. 2, 160 (2004).

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited