In single-particle tracking (SPT), fluorescence video microscopy is used to record the motion images of single particle or single molecule. Here, by using a total-internal-reflection microscope equipped with an argon ion laser and a charge-coupled device (CCD) camera with high-speed and high-sensitivity, video images of single nanobeads in solutions were obtained. From the trajectories, the diffusion coefficient of individual nanobead was determined by the mean square displacements as a function of time. The sizes of nanobeads were calculated by Stokes-Einstein equation, and the results were compared with the actual values.
© 2005 Chinese Optics Letters
Qin Yu, Chen Wang, Li Liu, and Guiying Wang, "Imaging and tracking single fluorescent nanobeads in solution," Chin. Opt. Lett. 3, S256-S258 (2005)