The dynamical equation of a trapping cell is solved to find calibration methods for the trapping force, and two methods are compared by synthetic experiment data. Results indicate that: Boltzmann distribution method (BDM) is available for the force calibration of non-spherical or anisotropic cells in arbitrary trap potential; the mean square displacement method (MSDM) is available only for a symmetric harmonic optical trap. The spatial resolution requirement of the calibration system is about a nanometer. The results agree with the reported experiments.
© 2006 Chinese Optics Letters
Youli Yu, Zhenxi Zhang, Zheng Li, and Xiaoli Wang, "Methods of calibration to optical trapping force upon non-spherical cells," Chin. Opt. Lett. 4, 722-724 (2006)