OSA's Digital Library

Chinese Optics Letters

Chinese Optics Letters


  • Vol. 4, Iss. 3 — Mar. 10, 2006
  • pp: 177–180

Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip

Honghua Hu, Xiyun Hou, and Guoguang Yang  »View Author Affiliations

Chinese Optics Letters, Vol. 4, Issue 3, pp. 177-180 (2006)

View Full Text Article

Acrobat PDF (265 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


The laser induced fluorescence (LIF) detection system for 5-um microspheres delivered on microfluidic chip is presented employing confocal optical scheme. The parameters of the optical system are specifically optimized for single microsphere detection. With the excitation laser spot size of 4.6 um and optical sectioning power of 27 um, the lowest concentration detection limit is 0.45 nmol/L, corresponding to only 122 molecules in probe volume. The microsphere detection is carried on successfully with the maximum signal-to-noise ratio (SNR) of 55.7, which provides good detection sensitivity.

© 2005 Chinese Optics Letters

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(350.4990) Other areas of optics : Particles

Honghua Hu, Xiyun Hou, and Guoguang Yang, "Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip," Chin. Opt. Lett. 4, 177-180 (2006)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. D. A. A. Vignali, Journal of Immunological Methods 243, 243 (2000).
  2. R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).
  3. F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).
  4. A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).
  5. LiquiChip(TM) Applications Handbook (2002).
  6. T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).
  7. J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).
  8. Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).
  9. H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).
  10. G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).
  11. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (in Chinese) G. Wang, Z. Chen, and L. Yang, (trans.) (New Times Press, Beijing, 2000).
  12. Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited