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Chinese Optics Letters

Chinese Optics Letters


  • Vol. 6, Iss. 12 — Dec. 1, 2008
  • pp: 882–885

Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen

Hanping Liu, Zhengfei Zhuang, Lingling Zhao, Junle Qu, and Xiaoyuan Deng  »View Author Affiliations

Chinese Optics Letters, Vol. 6, Issue 12, pp. 882-885 (2008)

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Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson's (VG) alcoholic picrofuchsin staining on SHG in type I collagen (from tendon-rich C57BL/6). Multi-channel imaging and spectra analysis show that the strong SHG signal produced in fresh collagen type I fiber has been greatly suppressed after VG staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline.

© 2008 Chinese Optics Letters

OCIS Codes
(170.6930) Medical optics and biotechnology : Tissue
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

Hanping Liu, Zhengfei Zhuang, Lingling Zhao, Junle Qu, and Xiaoyuan Deng, "Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen," Chin. Opt. Lett. 6, 882-885 (2008)

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