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Chinese Optics Letters

Chinese Optics Letters


  • Vol. 6, Iss. 12 — Dec. 1, 2008
  • pp: 938–940

Micro-Raman spectroscopy of single leukemic cells

Changmei Cai, Rong Chen, Juqiang Lin, Yongzeng Li, and Shangyuan Feng  »View Author Affiliations

Chinese Optics Letters, Vol. 6, Issue 12, pp. 938-940 (2008)

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The Raman spectra from leukemic cell line (HL60) and normal human peripheral blood mononuclear cells (PBMCs) are obtained by confocal micro-Raman spectroscopy using near-infrared laser (785 nm) excitation. The scanning range is from 500 to 2000 cm^{-1}. The two average Raman spectra of normal PBMCs and carcinoma cells have clear differences because their structure and amount of nucleic acid, protein, and other major molecules are changed. The spectra are also compared and analyzed by principal component analysis (PCA) to demonstrate the two distinct clusters of normal and transformed cells. The sensitivity of this technique for identifying transformed cells is 100%.

© 2008 Chinese Optics Letters

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy
(300.6450) Spectroscopy : Spectroscopy, Raman

Changmei Cai, Rong Chen, Juqiang Lin, Yongzeng Li, and Shangyuan Feng, "Micro-Raman spectroscopy of single leukemic cells," Chin. Opt. Lett. 6, 938-940 (2008)

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