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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 6, Iss. 12 — Dec. 1, 2008
  • pp: 944–946

Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME

Huijuan Yin, Yingxin Li, Zhaohui Zou, Wentao Qiao, Xue Yao, Yang Su, and Hongyan Guo  »View Author Affiliations


Chinese Optics Letters, Vol. 6, Issue 12, pp. 944-946 (2008)


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Abstract

To investigate the effect of photodynamic therapy (PDT) with hematoporphrin monomethyl ether (HMME) on bovine immunodeficiency virus (BIV) can provide the basis theory for photoinactivation of human immunodeficiency virus (HIV). To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-(4,5)-dimethylthiahiazol-2-y1-3,5-di-phenytetrazolium bromide (MTT) with power density of 5 and 25 mW/cm2 and energy density from 0.6 to 3 J/cm2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.

© 2008 Chinese Optics Letters

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5180) Medical optics and biotechnology : Photodynamic therapy

Citation
Huijuan Yin, Yingxin Li, Zhaohui Zou, Wentao Qiao, Xue Yao, Yang Su, and Hongyan Guo, "Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME," Chin. Opt. Lett. 6, 944-946 (2008)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-6-12-944

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