OSA's Digital Library

Chinese Optics Letters

Chinese Optics Letters


  • Vol. 7, Iss. 3 — Mar. 1, 2009
  • pp: 240–243

Nonlinear spectral imaging microscopy of rabbit aortic wall

Quangang Liu, Jianxin Chen, Shuangmu Zhuo, Xingshan Jiang, and Kecheng Lu  »View Author Affiliations

Chinese Optics Letters, Vol. 7, Issue 3, pp. 240-243 (2009)

View Full Text Article

Acrobat PDF (2317 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


Employing nonlinear spectral imaging technique based on two-photon-excited fluorescence and second-harmonic generation (SHG) of biological tissue, we combine the image-guided spectral analysis method and multi-channel subsequent detection imaging to map and visualize the intrinsic species in a native rabbit aortic wall. A series of recorded nonlinear spectral images excited by a broad range of laser wavelengths (730-910 nm) are used to identify five components in the native rabbit aortic wall, including nicotinamide adenine dinucleotide (NADH), elastic fiber, flavin, porphyrin derivatives, and collagen. Integrating multi-channel subsequent detection imaging technique, the high-resolution, high contrast images of collagen and elastic fiber in the aortic wall are obtained. Our results demonstrate that this method can yield complementary biochemical and morphological information about aortic tissues, which have the potential to determine the tissue pathology associated with mechanical properties of aortic wall and to evaluate the pharmacodynamical studies of vessels.

© 2009 Chinese Optics Letters

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6410) Spectroscopy : Spectroscopy, multiphoton

Quangang Liu, Jianxin Chen, Shuangmu Zhuo, Xingshan Jiang, and Kecheng Lu, "Nonlinear spectral imaging microscopy of rabbit aortic wall," Chin. Opt. Lett. 7, 240-243 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. G. Boyer and K. Plamann, Chin. Opt. Lett. 5, 477 (2007).
  2. S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).
  3. S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).
  4. P. J. Campagnola and L. M. Loew, Nature Biotechnol. 21, 1356 (2003).
  5. S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).
  6. S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).
  7. S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).
  8. R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996).
  9. J. Chen, S. Zhuo, T. Luo, and J. Zhao, Chin. Opt. Lett. 4, 598 (2006).
  10. J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).
  11. J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).
  12. S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).
  13. J. A. Palero, H. S. de Bruijn, A. van der Ploeg-van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen, Opt. Express 14, 4395 (2006).
  14. J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).
  15. S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).
  16. T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited