OSA's Digital Library

Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 8, Iss. 10 — Oct. 1, 2010
  • pp: 934–936

Improving the precision of fluorescence lifetime measurement using a streak camera

Heng Li, Yonghong Shao, Yan Wang, Junle Qu, and Hanben Niu  »View Author Affiliations


Chinese Optics Letters, Vol. 8, Issue 10, pp. 934-936 (2010)


View Full Text Article

Acrobat PDF (384 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a twophoton excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. A continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a Fabry-Perot etalon is used to calibrate the nonlinearity of the sweeping speed. Fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision of the system, as characterized through the fluorescence lifetime of the short-lived fluorescence dye, Rose Bengal. Experimental results show that the measurement fluctuation of the lifetime has been improved from more than 10% to 2% after correcting the effects of gain nonuniformity and sweeping speed nonlinearity.

© 2010 Chinese Optics Letters

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(320.7080) Ultrafast optics : Ultrafast devices

Citation
Heng Li, Yonghong Shao, Yan Wang, Junle Qu, and Hanben Niu, "Improving the precision of fluorescence lifetime measurement using a streak camera," Chin. Opt. Lett. 8, 934-936 (2010)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-8-10-934


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. W. Denk, D. W. Piston, and W. W. Webb, in Handbook of Biological Confocal Microscopy J. B. Pawley, (ed.) (Plenum Press, New York, 1995)p. 445.
  2. S. Weiss, Science 283, 1676 (1999).
  3. B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).
  4. R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).
  5. H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).
  6. H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).
  7. D. K. Bird, K. W. Eliceiri, C.-H. Fan, and J. G. White, Appl. Opt. 43, 5173 (2004).
  8. H. Brismar and B. Ulfhake, Nature Biotechnol. 15, 373(1997).
  9. Q. S. Hanley, D. J. Arndt-Jovin, and T. M. Jovin, Appl. Spectrosc. 56, 155 (2002).
  10. H. Wallrabe and A. Periasamy, Current Opinion in Biotechnol. 16, 19 (2005).
  11. V. E. Gaviola, Zeitschrift f¨ur Physik A Hadrons and Nuclei (in German) 42, 853 (1927).
  12. R. D. Spencer and G. Weber, Ann. NY Acad. Sci. 158, 361 (1969).
  13. W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).
  14. K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).
  15. R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).
  16. L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).
  17. Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).
  18. http://www.iss.com/resources/lifetime.html.(September 11, 2010)

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited