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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 8, Iss. 10 — Oct. 1, 2010
  • pp: 944–946

A miniature laser speckle fluorescence sectioning microscope for cell imaging

Yonghong Shao, Heng Li, Qiao Wen, Yan Wang, Junle Qu, and Hanben Niu  »View Author Affiliations


Chinese Optics Letters, Vol. 8, Issue 10, pp. 944-946 (2010)


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Abstract

We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (DPSS) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary artery endothelial (BPAE) cells are obtained. The image quality is similar to that of the images acquired by standard laser scanning confocal microscope (LSCM). Compared with LSCM, the laser speckle fluorescence microscope (LSFM) presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.

© 2010 Chinese Optics Letters

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
Yonghong Shao, Heng Li, Qiao Wen, Yan Wang, Junle Qu, and Hanben Niu, "A miniature laser speckle fluorescence sectioning microscope for cell imaging," Chin. Opt. Lett. 8, 944-946 (2010)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-8-10-944

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