Bim, a proapoptotic member of Bcl-2 family, plays an important role in cell apoptosis. It is generally thought that Bim translocates to mitochondria in response to apoptotic stimuli. We use confocal microscopy to image the temporal-spatial dynamics of Bim during dihydroartemisinin (DHA) induced human lung adenocarcinoma (ASTC-a-1) cell apoptosis. Interestingly, we find that DHA induces Bim translocation to endoplasmic reticulum (ER) rather than mitochondria, implying that Bim-ER pathway might be involved in the DHA-induced ASTC-a-1 cell apoptosis.
© 2010 Chinese Optics Letters
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
Min Chen, Yingying Lu, Tongsheng Chen, and Junle Qu, "Confocal imaging of Bim translocation to endoplasmic reticulum during DHA-induced ASTC-a-1 cell apoptosis," Chin. Opt. Lett. 8, 950-952 (2010)