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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 8, Iss. 10 — Oct. 1, 2010
  • pp: 953–956

Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry

Yan Li, Zhichao Fan, Jin Guo, Guangda Liu, Xiaoying Tan, Cheng Wang, Zhengqin Gu, Xunbin Wei, and  »View Author Affiliations


Chinese Optics Letters, Vol. 8, Issue 10, pp. 953-956 (2010)


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Abstract

Hepatocellular carcinoma (HCC) may metastasize to many organs. The survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed. We have developed the "in vivo microscopy" to study the mechanisms that govern liver tumor cells spreading through the microenvironment in vivo. A recently developed "in vivo flow cytometer" combined with real-time confocal fluorescence imaging is used to assess spreading and the circulation kinetics of liver tumor cells. We measure the depletion kinetics of two related human HCC cell lines, high-metastatic HCCLM3 cells and low-metastatic HepG2 cells, which are from the same origin and obtained by repetitive screenings in mice. More than 60% of the HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, less than 40% of the HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.

© 2010 Chinese Optics Letters

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

Citation
Yan Li, Zhichao Fan, Jin Guo, Guangda Liu, Xiaoying Tan, Cheng Wang, Zhengqin Gu, Xunbin Wei, and , "Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry," Chin. Opt. Lett. 8, 953-956 (2010)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-8-10-953


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References

  1. E. Bedner, H. D. Halicka, W. Cheng, T. Salomon, A. Deptala, W. Gorczyca, M. R. Melamed, and Z. Darzynkiewicz, Cytometry 36, 77 (1999).
  2. F. G. Blankenberg, L. Naumovski, J. F. Tait, A. M. Post, and H. W. Strauss, J. Nucl. Medicine 42, 309 (2001).
  3. T. Aotake, C. D. Lu, Y. Chiba, R. Muraoka, and N. Tanigawa, Clin. Cancer Res. 5, 135 (1999).
  4. F. Durrieu, F. Belloc, L. Lacoste, P. Dumain, J. Chabrol, J. Dachary-Prigent, H. Morjani, M. R. Boisseau, J. Reiffers, P. Bernard, and F. Lacombe, Exp. Cell Res. 240, 165 (1998).
  5. O. Ek, G. H. Reaman, D. L. Crankshaw, L. M. Chelstrom, D. E. Myers, and F. M. Uckun, Leuk. Lymphoma 28, 509 (1998).
  6. N. Gaiano, J. S. Nye, and G. Fishell, Neuron 26, 395(2000).
  7. N. Gaiano, J. D. Kohtz, D. H. Turnbull, and G. Fishell, Nature Neurosci. 2, 812 (1999).
  8. B. Fadeel, S. Orrenius, and B. Zhivotovsky, Biochem. Biophys. Res. Commun. 266, 699 (1999).
  9. I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, Cancer Res. 64, 5044 (2004).
  10. H. R. Herschman, Science 302, 605 (2003).
  11. S. Holdenrieder and P. Stieber, Clin. Biochem. 37, 605(2004).
  12. S. Jahr, H. Hentze, S. Englisch, D. Hardt, F. O. Fackelmayer, R. D. Hesch, and R. Knippers, Cancer Res. 61, 1659 (2001).
  13. B. Laxman, D. E. Hall, M. S. Bhojani, D. A. Hamstra, T. L. Chenevert, B. D. Ross, and A. Rehemtulla, PNAS 99, 16551 (2002).
  14. T. F. Massoud and S. S. Gambhir, Genes Development 17, 545 (2003).
  15. S. Nery, H. Wichterle, and G. Fishell, Development 128, 527 (2001).
  16. V. Ntziachristos, E. A. Schellenberger, J. Ripoll, D. Yessayan, E. Graves, A. Bogdanov, Jr., L. Josephson, and R. Weissleder, PNAS 101, 12294 (2004).
  17. S. Osella-Abate, A. Zaccagna, P. Savoia, P. Quaglino, B. Salomone, and M. G. Bernengo, J. Am. Acad. Dermatol. 44, 40 (2001).
  18. J. C. Reed, J. Clin. Oncol. 17, 2941 (1999).
  19. L. Li, H. Wei, B. He, Z. Guo, H. Yang, S. Xie, X. Chen, L. Li, G. Wu, and J. Lu, Acta Opt. Sin. (in Chinese) 29, 2860 (2009).
  20. J. Li, H. Zeng, and X. Pang, Acta Opt. Sin. (in Chinese) 29, 2261 (2009).
  21. H. Zhang, W. Ma, Z. Zou, W. Gao, Z. Xue, and Y. Li, Chinese J. Lasers (in Chinese) 35, 631 (2008).
  22. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004).
  23. S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. Lin, and I. Georgakoudi, J. Biomed. Opt. 12,020507 (2007).
  24. D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Cote, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, Nature 435, 969 (2005).
  25. J. Novak and M. Puoris'haag, Opt. Lett. 32, 2993(2007).
  26. W. He, H. F. Wang, L. C. Hartmann, J. X. Cheng, and P. S. Low, PNAS 104, 11760 (2007).
  27. C. F. Zhong, E. R. Tkaczyk, T. Thomas, J. Y. Ye, A. Myc, A. U. Bielinska, Z. Cao, I. Majoros, B. Keszler, J. R. Baker, and T. B. Norris, J. Biomed. Opt. 13, 59801(2008).
  28. Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, Nature Medicine 16, 718 (2010).
  29. Y. Li, Z. Y. Tang, S. L. Ye, B. B. Liu, Y. K. Liu, J. Chen, and Q. Xue, J. Cancer Res. Clin. Oncol. 129, 43 (2003).
  30. X. Wei, D. A. Sipkins, C. M. Pitsillides, J. Novak, I. Georgakoudi, and C. P. Lin, Mol. Imaging 4, 415 (2005).
  31. E. R. Tkaczyk, C. F. Zhong, J. Y. Ye, S. Katnik, A. Myc, K. E. Luker, G. D. Luker, J. R. Baker, and T. B. Norris, Proc. SPIE 6631, 66310T (2007).
  32. E. R. Tkaczyk, A. H. Tkaczyk, S. Katnik, J. Y. Ye, K. E. Luker, G. D. Luker, A. Myc, J. R. Baker, and T. B. Norris, J. Biomed. Opt. 13, 041319 (2008).
  33. C. F. Zhong, J. Y. Ye, A. Myc, T. P. Thomas, A. U. Bielinska, J. R. Baker, Jr., and T. B. Norris, Proc. SPIE 5700, 78 (2005).
  34. E. R. Tkaczyk, C. F. Zhong, J. Y. Ye, A. Myc, T. Thomas, Z. Cao, R. Duran-Struuck, K. E. Luker, G. D. Luker, T. B. Norris, and J. R. Baker, Jr., Opt. Commun. 281, 888(2008).
  35. Y. C. Chang, J. Y. Ye, T. P. Thomas, Z. Cao, A. Kotlyar, E. R. Tkaczyk, J. R. Baker, Jr., and T. B. Norris, J. Biomed. Opt. 15, 047004 (2010).
  36. V. P. Zharov, E. I. Galanzha, and V. V. Tuchin, Opt. Lett. 30, 628 (2005).
  37. V. P. Zharov, E. I. Galanzha, and V. V. Tuchin, J. Cell. Biochem. 97, 916 (2006).
  38. V. P. Zharov, E. I. Galanzha, E. V. Shashkov, N. G. Khlebtsov, and V. V. Tuchin, Opt. Lett. 31, 3623(2006).
  39. E. I. Galanzha, E. V. Shashkov, V. V. Tuchin, and V. P. Zharov, Cyrometry Part A 73, 884(2008).
  40. E. I. Galanzha, M. S. Kokoska, E. V. Shashkov, J. W. Kim, V. V. Tuchin, and V. P. Zharov, J. Biophoton. 2, 528 (2009).
  41. E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim,L. Yang, and V. P. Zharov, Nature Nanotechnol. 4, 855(2009).

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