The action mode between Dy(III)(NR)3 and herring sperm DNA is studied by ultraviolet-visible (UV-vis) and fluorescence spectra as well as electrochemistry. Double-reciprocal method studies show that the binding stoichiometry between Dy(III)(NR)3 and DNA is 1:1, the binding constants at different temperatures are 1.15×105 L/mol at 25 °C and 2.09×105 L/mol at 35 °C, and the corresponding thermodynamic parameters are ΔrH Θm = 2.48×104 J/mol, ΔrG Θm =-2.34×104 J/mol, ΔrSm=161.7 J/(mol·K); ΔrS Θm is the driving force in this reaction. Combined with Scatchard method and melting method, the results suggest that the interaction mode between Dy(III)(NR)3 and herring sperm DNA is intercalation fashion and groove fashion.
© 2010 Chinese Optics Letters
Yan Zhang, Xingming Wang, Dan Fei, Na Zhao, Tingting Zhao, Haifeng Zhu, and Lisheng Ding, "Study on interaction between Dy(III)(NR)3 complex and herring sperm DNA by spectroscopy," Chin. Opt. Lett. 8, 236-240 (2010)