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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 9, Iss. 2 — Feb. 10, 2011
  • pp: 022801–

Immunosensor application based on Raman spectroscopy of porous silicon

Tao Jiang, Jiaqing Mo, Xiaoyi Lv, Pengfei Yan, Zhenhong Jia, Jiangwei Li, and Fuchun Zhang  »View Author Affiliations


Chinese Optics Letters, Vol. 9, Issue 2, pp. 022801- (2011)


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Abstract

Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A (spaA) of below 4.0 ?g·ml?1. The ultimate detection limit is 1.412×10^2 pg.ml^{?1}. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free.

© 2011 Chinese Optics Letters

OCIS Codes
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(070.4790) Fourier optics and signal processing : Spectrum analysis
(280.0280) Remote sensing and sensors : Remote sensing and sensors
(280.1415) Remote sensing and sensors : Biological sensing and sensors

Citation
Tao Jiang, Jiaqing Mo, Xiaoyi Lv, Pengfei Yan, Zhenhong Jia, Jiangwei Li, and Fuchun Zhang, "Immunosensor application based on Raman spectroscopy of porous silicon," Chin. Opt. Lett. 9, 022801- (2011)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-9-2-022801


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