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Chinese Optics Letters

Chinese Optics Letters

| PUBLISHED MONTHLY BY CHINESE LASER PRESS AND DISTRIBUTED BY OSA

  • Vol. 9, Iss. 3 — Mar. 10, 2011
  • pp: 030901–

Non-invasive monitoring of living cell culture by lensless digital holography imaging

Yunxin Wang, Dayong Wang, Jie Zhao, Yishu Yang, Xiangqian Xiao, and Huakun Cui  »View Author Affiliations


Chinese Optics Letters, Vol. 9, Issue 3, pp. 030901- (2011)


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Abstract

A non-invasive detection method for the status analysis of cell culture is presented based on digital holography technology. Lensless Fourier transform digital holography (LFTDH) configuration is developed for living cell imaging without prestaining. Complex amplitude information is reconstructed by a single inverse fast Fourier transform, and the phase aberration is corrected through the two-step phase subtraction method. The image segmentation is then applied to the automatic evaluation of confluency. Finally, the cervical cancer cell TZMbl is employed for experimental validation, and the results demonstrate that LFTDH imaging with the corresponding image post-processing can provide an automatic and non-invasive approach for monitoring living cell culture.

© 2011 Chinese Optics Letters

OCIS Codes
(090.0090) Holography : Holography
(100.0100) Image processing : Image processing
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

Citation
Yunxin Wang, Dayong Wang, Jie Zhao, Yishu Yang, Xiangqian Xiao, and Huakun Cui, "Non-invasive monitoring of living cell culture by lensless digital holography imaging," Chin. Opt. Lett. 9, 030901- (2011)
http://www.opticsinfobase.org/col/abstract.cfm?URI=col-9-3-030901

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