OSA's Digital Library

Journal of Lightwave Technology

Journal of Lightwave Technology


  • Vol. 31, Iss. 8 — Apr. 15, 2013
  • pp: 1205–1210

Continuous Measurement of Particle Depth in a Microchannel Using Chromatic Aberration

Shin-Yu Su and Che-Hsin Lin

Journal of Lightwave Technology, Vol. 31, Issue 8, pp. 1205-1210 (2013)

View Full Text Article

Acrobat PDF (876 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


Detecting the z-position of moving objects in an embedded microchannel is an important but highly challenging problem in the MEMS field. The present study proposes a new depth measurement system based on the chromatic aberration effect under a dark-field illumination scheme. The microchannel is illuminated by dispersed white light and the light scattered from the moving objects is captured by a low numerical aperture (N.A.) objective lens. Due to chromatic aberration effect, sample in various positions will scatter different wavelengths. The depth of each moving object is then determined by inspecting the intensity ratio of the scattered spectral components with wavelengths of 450 nm (blue light) and 670 nm (red light), respectively. Experimental results show that the proposed system enables the object depth to be measured over a range of ±15 μm while using acrylic lens for light aberration. Alternatively, the developed system is capable to discriminate the depth change of 2 μm micro-beads when a higher Abbe number material of BK7 lens is used for light aberration. The depth measurements are obtained without the need for a delicate optical system or scanning process with the developed system. The use of UV-Vis-NIR spectrometer enables this system to analyze the depths of the samples in flow velocity 500 μm/sec. The proposed system provides a straightforward yet highly effective means of determining the depth of moving objects in microfluidic channels in a continuous manner.

© 2013 IEEE

Shin-Yu Su and Che-Hsin Lin, "Continuous Measurement of Particle Depth in a Microchannel Using Chromatic Aberration," J. Lightwave Technol. 31, 1205-1210 (2013)

Sort:  Year  |  Journal  |  Reset


  1. A. van den Berg, T. S. J. Lammerink, "Micro total analysis systems: Microfluidic aspects, integration concept and applications," Microsyst. Technol. Chem. Life Sci. 194, 21-49 (1998).
  2. T. Vilkner, D. Janasek, A. Manz, "Micro total analysis systems. recent developments," Anal. Chem. 76, 3373-3385 (2004).
  3. J. K. A. Nicholson, W. M. Velleca, S. Jubert, T. A. Green, L. Bryan, "Evaluation of alternative Cd4 technologies for the enumeration of Cd4 lymphocytes," J. Immunolog. Meth. 177, 43-54 (1994).
  4. H. Radtke, S. Lindenau, B. Holbach, S. Neumann, H. Kiesewetter, "Flow cytometry for white blood-cell counts in whole-blood and blood components," Infusionstherapie Und Transfusionsmedizin 22, 112-114 (1995).
  5. S. Bottcher, M. Ritgen, M. Bruggemann, T. Raff, S. Luschen, A. Humpe, M. Kneba, C. Pott, "Flow cytometric assay for detennination of Fc gamma RIIIA-158 V/F polymorphism," J. Immunolog. Meth. 306, 128-136 (2005).
  6. E. Dekking, V. H. J. van der Velden, S. Bottcher, M. Bruggemann, E. Sonneveld, A. Koning-Goedheer, N. Boeckx, P. Lucio, L. Sedek, T. Szczepanski, T. Kalina, M. Kovac, P. Evans, P. G. Hoogeveen, J. Flores-Montero, A. Orfao, W. M. Comans-Bitter, F. J. T. Staal, J. J. M. van Dongen, "Detection of fusion genes at the protein level in leukemia patients via the flow cytometric immunobead assay," Best Practice Res. Clin. Haematol. 23, 333-345 (2010) E. C. E. FP6, L. C. 018708.
  7. C. H. Lin, G. B. Lee, "Micromachined flow cytometers with embedded etched optic fibers for optical detection," J. Micromech. Microeng. 13, 447-453 (2003).
  8. X. Li, M. R. Melamed, Z. Darzynkiewicz, "Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color," Experimental Cell Res. 222, 28-37 (1996).
  9. H. C. Lee, H. H. Hou, R. J. Yang, C. H. Lin, L. M. Fu, "Microflow cytometer incorporating sequential micro-weir structure for three-dimensional focusing," Microfluid. Nanofluid. 11, 469-478 (2011).
  10. H. Klank, G. Goranovic, J. P. Kutter, H. Gjelstrup, J. Michelsen, C. H. Westergaard, "PIV measurements in a microfluidic 3D-sheathing structure with three-dimensional flow behaviour," J. Micromech. Microeng. 12, 862-869 (2002).
  11. S. Y. Yoon, K. C. Kim, "3D particle position and 3D velocity field measurement in a microvolume via the defocusing concept," Meas. Sci. Technol. 17, 2897-2905 (2006).
  12. S. Choi, S. H. Kim, J. K. Park, "Optical path-length modulation for three-dimensional particle measurement in mirror-embedded microchannels," Lab on a Chip 10, 335-340 (2010).
  13. R. Lima, S. Wada, K. Tsubota, T. Yamaguchi, "Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel," Meas. Sci. Technol. 17, 797-808 (2006).
  14. K. B. Shi, P. Li, S. Z. Yin, Z. W. Liu, "Chromatic confocal microscopy using supercontinuum light," Opt. Exp. 12, 2096-2101 (2004).
  15. B. S. Chun, K. Kim, D. Gweon, "Three-dimensional surface profile measurement using a beam scanning chromatic confocal microscope," Rev. Sci. Instrum. 80, (2009).
  16. P. C. Lin, P. C. Sun, L. J. Zhu, Y. Fainman, "Single-shot depth-section imaging through chromatic slit-scan confocal microscopy," Appl. Opt. 37, 6764-6770 (1998).
  17. R. Duplov, "Apochromatic telescope without anomalous dispersion glasses," Appl. Opt. 45, 5164-5167 (2006).
  18. S. W. Lin, J. H. Hsu, C. H. Chang, C. H. Lin, "Objective-type dark-field system applied to multi-wavelength capillary electrophoresis for fluorescent detection and analysis," Biosens. Bioelectron. 25, 450-455 (2009).
  19. E. Hecht, Optics (Addison-Wesley, 2002).
  20. T. Matsuda, Y. Funae, M. Yoshida, T. Yamamoto, T. Takaya, "Optical material of high refractive index resin composed of sulfur-containing aromatic methacrylates," J. Appl. Polymer Sci. 76, 50-54 (2000).
  21. C. H. Lin, G. B. Lee, Y. H. Lin, G. L. Chang, "A fast prototyping process for fabrication of microfluidic systems on soda-lime glass," J. Micromechan. Microeng. 11, 726-732 (2001).

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited