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Journal of the Optical Society of America

Journal of the Optical Society of America

  • Vol. 58, Iss. 5 — May. 1, 1968
  • pp: 661–664

Tandem-Scanning Reflected-Light Microscope


JOSA, Vol. 58, Issue 5, pp. 661-664 (1968)

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Reflected-light microscopy of semitransparent material, such as unstained nervous tissue, is usually unsatisfactory because of low contrast and light scattering. In a new microscope both the object plane and the image plane were scanned in tandem so that only light reflected from the object plane was included in the image. The object was illuminated with nearly incoherent light passing through holes in one side of a rotating scanning disk (Nipkow wheel) which was imaged by the objective into the object plane. Reflected-light images of these spots were conducted to the opposite side of the same disk. Light could pass from the source to the object plane, and from the object to the image plane, only through optically congruent holes on opposite side of the rotating disk. The image obtained had better contrast and sharpness for some semi-transparent material than possible in usual reflected-light microscopy.

MOJMÍR PETRÁŇ, MILAN HADRAVSKÝ, M. DAVID EGGER, and ROBERT GALAMBOS, "Tandem-Scanning Reflected-Light Microscope," J. Opt. Soc. Am. 58, 661-664 (1968)

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  1. An extension of the theory given here indicates that a similar principal could be employed to build an optical microscope with a resolving power greater than that predicted by the Rayleigh and Abbe theories (superresolution in the sense of Herzberger). Such a microscope would be related to the system described theoretically, from another point of view, by W. Lukosz and M. Marchand, Opt. Acta 10, 241 (1963); see also W. Lukosz, J. Opt. Soc. Am. 56, 1463 (1966); A. Bachl and W. Lukosz, J. Opt. Soc. Am. 57, 932 (1967).
  2. M. Petráň and M. Hadravský, Czechoslovakian patent appl. 7720, 1966.
  3. Perfected by Zeiss. Cited by M. Françon, Progress in Microscopy (Row, Peterson and Co., Evanston, Ill., 1961), p. 131.
  4. A disk of tantalum carbide or hafnium carbide excited by radio frequency would provide a better source of light for our purpose. S. C. Peek, Illumin. Eng. 52, 96 (1957).
  5. M. D. Egger and M. Petráň, Science 157, 305 (1967).

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