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Journal of the Optical Society of America

Journal of the Optical Society of America

  • Vol. 40, Iss. 5 — May. 1, 1950
  • pp: 295–303

Microscope Imagery and Interpretations

HAROLD OSTERBERG  »View Author Affiliations

JOSA, Vol. 40, Issue 5, pp. 295-303 (1950)

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The physical limits of resolution are discussed for the following basic types of object specimens: periodic structures which exert no focusing action; periodic structures which do exert focusing action upon the incident light; two pin holes or two small particles against opaque or transmitting backgrounds; two narrow slits or object strips against opaque or transmitting backgrounds; and two, sub-microscopic, self-luminous particles. Abbe’s theory remains valid as applied to periodic structures. Generalized resolution formulas are given for periodic structures. Graphical data, based upon recent analysis, show that the resolution of two particles or slits is more complex than indicated by classical theory. The resolving power of the microscope is underestimated by the classical theory. Abnormally high resolutions can occur with properly chosen particles. The class of particles for which the phase microscope has superlative resolving power is described.

HAROLD OSTERBERG, "Microscope Imagery and Interpretations," J. Opt. Soc. Am. 40, 295-303 (1950)

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  1. R. K. Luneberg, Mathematical Theory of Optics (Brown University, Providence, 1944), p. 364.
  2. Ramsay, Cleveland, and Koppius, J. Opt. Soc. Am. 31, 28 (1941).
  3. R. K. Luneberg, reference 1, p. 390.
  4. H. Osterberg and F. C. Wissler, J. Opt. Soc. Am. 39, 561 (1949).
  5. H. Osterberg and F. C Wissler, J. Opt, Soc, Am, 39, 565 (1949).

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