OSA's Digital Library

Journal of the Optical Society of America

Journal of the Optical Society of America

  • Vol. 40, Iss. 5 — May. 1, 1950
  • pp: 329–334

Color Phase-Contrast Microscopy: Requirements and Applications

CHARLES PROFFER SAYLOR, ARTHUR T. BRICE, and F. ZERNIKE  »View Author Affiliations


JOSA, Vol. 40, Issue 5, pp. 329-334 (1950)
http://dx.doi.org/10.1364/JOSA.40.000329


View Full Text Article

Acrobat PDF (1099 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

For the use of color phase contrast in microscopy, which was first discussed in the Spring of 1948, several difficult requirements must be met for the attainment of maximum brilliance and usefulness. The system itself is one in which a controlled chromatism of the phase plate is sought instead of the customary control of phase difference at one wave-length only. The thickness and dispersion of the phase ring in relation to the general aperture can be such that zero phase contrast will occur at one or more wave-lengths of transition in color sensation. If the phase contrast is made high but opposite in several spectral zones of relatively pure color sensation, striking and beautiful microscopical images are formed with objects of low inherent contrast. The greatest advantage of the system lies not in the beauty of the images, but in the clear distinction which is afforded between effects caused by scattering or general absorption and those caused by small differences of refractive index or thickness.

Citation
CHARLES PROFFER SAYLOR, ARTHUR T. BRICE, and F. ZERNIKE, "Color Phase-Contrast Microscopy: Requirements and Applications," J. Opt. Soc. Am. 40, 329-334 (1950)
http://www.opticsinfobase.org/josa/abstract.cfm?URI=josa-40-5-329

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited