OSA's Digital Library

Journal of the Optical Society of America

Journal of the Optical Society of America

  • Vol. 51, Iss. 12 — Dec. 1, 1961
  • pp: 1366–1372

Spectral Transmission and Scattering Properties of Red Blood Cells


JOSA, Vol. 51, Issue 12, pp. 1366-1372 (1961)

View Full Text Article

Acrobat PDF (802 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Spectral curves of direct transmittance, diffuse transmittance, and scattering by partial layers of oriented red blood cells were measured. Also determined were the average optical parameters of a single cell and the cell concentration in the partial layers. The experiments are an extension and refinement of those of Lothian and Lewis on red blood cells and of Latimer on the green alga Chlorella. By assuming large-particle optics, equations were derived to predict the spectral curves in terms of cell and layer parameters. Experimental and theoretical curves are in reasonable agreement.

ROBERT A. MACRAE, JOSEPH A. MCCLURE, and PAUL LATIMER, "Spectral Transmission and Scattering Properties of Red Blood Cells," J. Opt. Soc. Am. 51, 1366-1372 (1961)

Sort:  Author  |  Journal  |  Reset


  1. E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.
  2. S. Anderson, J. Opt. Soc. Am. 39, 49 (1949).
  3. J. W. Weigl, J. Chem. Phys. 24, 577 (1956).
  4. P. Latimer and E. Rabinowitch, Arch. Biochem. Biophys. 84, 428 (1959).
  5. P. Latimer, Plant Physiol. 34, 193 (1959). See also, P. Latimer, Science 127, 29 (1958).
  6. F. S. Brackett and E. Charney, J. Opt. Soc. Am. 50, 811 (1960). See also E. Charney and F. Brackett, Arch. Biochem. Biophys. 92, 1 (1961).
  7. G. F. Lothian and P. C. Lewis, Nature 178, 1342 (1956). See also G. F. Lothian, Absorption Spectrophotometry (The Mac-Millan Company, New York, 1958), p. 35.
  8. P. Latimer, thesis, University of Illinois, Urbana, Illinois (1956).
  9. K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).
  10. S. Keilin and E. F. Hartree, Biochim. et Biophys. Acta 27, 173 (1958).
  11. M. Born and E. Wolf, Principles of Optics (Pergamon Press, New York, 1959), p. 395.
  12. H. C. van de Hulst, Light Scattering by Small Particles (John Wiley & Sons, Inc., New York, 1957), p. 107 and 193.
  13. P. Latimer, J. Opt. Soc. Am. 51, 116 (1961).
  14. M. M. Wintrobe, Clinical Hematology (Lea & Febiger Publishers, Philadelphia, Pennsylvania, 1951), 3rd ed., p. 312.
  15. R. Barer, Physical Techniques in Biological Research, III, edited by G. Oster and A. W. Pollister (Academic Press, Inc., New York, 1956), p. 29.
  16. D. L. Drabkin and J. H. Austin, J. Biol. Chem. 112, 51 (1951).
  17. R. Barer and S. Joseph, Quart. J. Microscop. Sci. 95, 399 (1954).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited