OSA's Digital Library

Journal of the Optical Society of America

Journal of the Optical Society of America

  • Vol. 54, Iss. 12 — Dec. 1, 1964
  • pp: 1451–1458

Sensitive Low-Light-Level Microspectrophotometer: Detection of Photosensitive Pigments of Retinal Cones

P. A. LIEBMAN and G. ENTINE  »View Author Affiliations

JOSA, Vol. 54, Issue 12, pp. 1451-1458 (1964)

View Full Text Article

Acrobat PDF (1608 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A microspectrophotometer of exceptional sensitivity has been constructed to record absorption spectra of visual pigments in single cells using minimum light. With this equipment, pigments of individual cones have been recorded in regions of less than 1 µ radius, with minimum bleaching. The instrument is simple and flexible in design, and uses commercially available components throughout. This paper reviews some general design considerations, the character and performance of our equipment, and some of the results obtained with retinal cones.

P. A. LIEBMAN and G. ENTINE, "Sensitive Low-Light-Level Microspectrophotometer: Detection of Photosensitive Pigments of Retinal Cones," J. Opt. Soc. Am. 54, 1451-1458 (1964)

Sort:  Author  |  Journal  |  Reset


  1. T. Caspersson, J. Roy. Microscop. Soc. 60, 8 (1940).
  2. B. Chance, R. Perry, L. Åkerman, and B. Thorell, Rev. Sci. Instr. 30, 735 (1959).
  3. P. Liebman. Biophys. J. 2, 161 (1962).
  4. R. A. Smith, Brit. J. Inst. Elect. Engr. 98, 43 (1951).
  5. C. C. Yang and V. Legallais, Rev. Sci. Instr. 25, 801 (1954).
  6. T. G. R. Rawlins, J. Opt. Soc. Am. 54, 423 (1964).
  7. G. P. Brouwer, Phillips Res. Rept. 18, 361 (1963).
  8. J. B. Johnson and R. B. Llewellyn, Bell Sys. Tech. J. 14, 85 (1935).
  9. W. B. Marks, Doctoral dissertation, Johns Hopkins University (1963).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited