Abstract

A microspectrophotometer of exceptional sensitivity has been constructed to record absorption spectra of visual pigments in single cells using minimum light. With this equipment, pigments of individual cones have been recorded in regions of less than 1 μ radius, with minimum bleaching. The instrument is simple and flexible in design, and uses commercially available components throughout. This paper reviews some general design considerations, the character and performance of our equipment, and some of the results obtained with retinal cones.

© 1964 Optical Society of America

Dichroic microspectrophotometer: A computer-assisted, rapid, wavelength-scanning photometer for measuring linear dichroism in single cells*

Ferenc I. Hárosi and Edward F. MacNichol
J. Opt. Soc. Am. 64(7) 903-918 (1974)

Validation of an Indicator of Mammalian Retinal Receptor Response: Action Spectrum*

Jay M. Enoch
J. Opt. Soc. Am. 54(3) 368-374 (1964)

Validation of an Indicator of Mammalian Retinal Receptor Response: Absolute Threshold *

Jay M. Enoch
J. Opt. Soc. Am. 54(8) 1027-1030 (1964)

Simplified Recording Microspectrophotometer

A. J. Casella, G. K. Strother, and J. W. Connolly
Appl. Opt. 14(3) 771-777 (1975)

Comparison of Reactions of Human and Rabbit Fundi to Photic Exposure

R. A. Weale
J. Opt. Soc. Am. 54(1) 120-126 (1964)