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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 15, Iss. 9 — Sep. 1, 1998
  • pp: 2275–2287

Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions

Ibrahim Akduman, Ulrich Brand, Jan Grochmalicki, Gerard Hester, Roy Pike, and Mario Bertero  »View Author Affiliations


JOSA A, Vol. 15, Issue 9, pp. 2275-2287 (1998)
http://dx.doi.org/10.1364/JOSAA.15.002275


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Abstract

A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA=1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.

© 1998 Optical Society of America

OCIS Codes
(070.4560) Fourier optics and signal processing : Data processing by optical means
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: January 27, 1998
Manuscript Accepted: May 4, 1998
Published: September 1, 1998

Citation
Ibrahim Akduman, Ulrich Brand, Jan Grochmalicki, Gerard Hester, Roy Pike, and Mario Bertero, "Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions," J. Opt. Soc. Am. A 15, 2275-2287 (1998)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-15-9-2275

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