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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 16, Iss. 9 — Sep. 1, 1999
  • pp: 2177–2184

Image enhancement through turbid media under a microscope by use of polarization gating methods

X. Gan, S. P. Schilders, and Min Gu  »View Author Affiliations


JOSA A, Vol. 16, Issue 9, pp. 2177-2184 (1999)
http://dx.doi.org/10.1364/JOSAA.16.002177


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Abstract

Image enhancement through a turbid medium by use of polarization gating methods in a microscopic imaging system is studied both theoretically and experimentally. A Monte Carlo simulation model based on Mie theory and geometric optics is adopted to calculate, for use with polarization gating and pinhole gating methods, image resolution of a sharp edge embedded in a turbid medium consisting of polystyrene beads. Both theoretical and experimental results show that polarization gating methods, particularly the differential polarization gating method, can be efficient in suppressing scattered photons, thus leading to image enhancement. In addition, a theoretical comparison of polarization gating and pinhole gating methods, based on the trade-off between resolution and signal strength, reveals that polarization gating methods are superior to the pinhole gating method when signal strength is weak.

© 1999 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.7050) Imaging systems : Turbid media

Citation
X. Gan, S. P. Schilders, and Min Gu, "Image enhancement through turbid media under a microscope by use of polarization gating methods," J. Opt. Soc. Am. A 16, 2177-2184 (1999)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-16-9-2177


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References

  1. M. Kempe, W. Rudolph, and E. Welsch, “Comparative study of confocal and heterodyne microscopy for imaging through scattering media,” J. Opt. Soc. Am. A 13, 46–52 (1996).
  2. D. Huang, E. A. Swanson, C. P. Lin, J. S Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flott, K. Gregory, C. A. Pulianfito, and J. G. Fujimoto, “Optical coherent tomography,” Science 254, 1178–1181 (1991).
  3. M. Toida, M. Kondo, T. Ichimura, and H. Inaba, “Two-dimensional coherent detection imaging in multiplescattering media based on the directional resolution capability of the optical heterodyne method,” Appl. Phys. B 52, 391–394 (1991).
  4. Q. Z. Wang, X. Liang, L. Wang, P. P. Ho, and R. R. Alfano, “Fourier spatial filter acts as a temporal gating for light propagating through a turbid medium,” Opt. Lett. 20, 1498–1501 (1995).
  5. J. M. Schmitt, A. Knüttel, and M. Yadlowsky, “Confocal microscopy in turbid media,” J. Opt. Soc. Am. A 11, 2226–2234 (1994).
  6. M. Gu, T. Tannous, and C. J. R. Sheppard, “Effect of an annular pupil on confocal imaging through highly scattering media,” Opt. Lett. 21, 312–314 (1996).
  7. X. Gan, S. Schilders, and M. Gu, “Combination of annular aperture and polarization gating methods for efficient microscopic imaging through a turbid medium: theoretical analysis,” Microsc. Microanal. 3, 495–503 (1997).
  8. S. Schilders, X. Gan, and M. Gu, “Microscopic imaging through turbid media by use of an annular objective for angle gating,” Appl. Opt. 37, 5320–5326 (1998).
  9. J. M. Schmitt, A. H. Gandjbakhche, and R. F. Bonner, “Use of polarization light to discriminate short path photons in a multiply scattering medium,” Appl. Opt. 31, 6535–6546 (1992).
  10. A. H. Hielscher, J. R. Mourant, and I. J. Bigio, “Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions,” Appl. Opt. 36, 125–135 (1997).
  11. S. G. Demos and R. R. Alfano, “Optical polarization imaging,” Appl. Opt. 36, 150–155 (1997).
  12. S. P. Morgan, M. P. Khong, and M. G. Somekh, “Effects of polarization state and scatter concentration on optical imaging through scattering media,” Appl. Opt. 36, 1560–1565 (1997).
  13. P. Bruscaglioni, G. Zaccaniti, and Q. Wei, “Transmission of a pulsed polarized light beam through thick turbid media: numerical results,” Appl. Opt. 32, 6142–6150 (1997).
  14. S. Schilders, X. Gan, and M. Gu, “Resolution improvement in microscopic imaging through turbid media based on differential polarization gating,” Appl. Opt. 37, 4300–4302 (1998).
  15. J. M. Schmitt and S. H. Xiang, “Cross-polarized backscatter in optical coherence tomography of biological tissue,” Opt. Lett. 23, 1160–1162 (1998).
  16. D. G. Papaioannou, G. W. Hooft, J. J. M. Baselman, and M. J. C. van Gemert, “Image quality in time-resolved transillumination of a highly scattering medium,” Appl. Opt. 34, 6144–6157 (1995).
  17. J. G. Fujimoto, S. De Silvestri, E. P. Ippen, C. A. Puliafito, R. Margolis, and A. Oseroff, “Femtosecond optical ranging in biological systems,” Opt. Lett. 3, 150–153 (1986).
  18. S. L Jacques, “Time resolved propagation of ultrashort laser pulses within turbid tissue,” Appl. Opt. 28, 2223–2229 (1989).
  19. F. C. Mackintosh, J. X. Zhu, D. J. Pine, and D. A. Weitz, “Polarization memory of multiply scattered light,” Phys. Rev. B 40, 9342–9345 (1989).
  20. K. M. Yoo and R. R. Alfano, “Time-resolved depolarization of multiple backscattered light from random media,” Phys. Lett. A 142, 531–536 (1989).
  21. X. Gan, S. P. Schilders, and M. Gu, “Image formation in turbid media under a microscope,” J. Opt. Soc. Am. A 15, 2052–2058 (1998).
  22. J. M. Schmitt and K. Ben-Letaief, “Efficient Monte Carlo simulation of confocal microscopy in biological tissue,” J. Opt. Soc. Am. A 13, 952–961 (1996).
  23. Y. Hasegawa, Y. Yamada, M. Tamura, and Y. Nomura, “Monte Carlo simulation of light transmission through living tissue,” Appl. Opt. 30, 4515–4520 (1991).
  24. H. Key, E. R. Davis, P. C. Jackson, and P. N. T. Wells, “Monte Carlo modeling of light propagation in breast tissue,” Phys. Med. Biol. 36, 591–602 (1991).
  25. M. Born and E. Wolf, Principles of Optics (Pergamon, New York, 1980).
  26. C. F. Bohern and D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).
  27. P. Török, P. Verga, Z. Laczik, and G. R. Booker, “Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integration representation,” J. Opt. Soc. Am. A 12, 325–332 (1996).
  28. S. P. Schilders, X. S. Gan, and M. Gu, “Effect of scatterer size on microscopic imaging through turbid media based on differential polarization-gating,” Opt. Commun. 157, 238–248 (1998).

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