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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 18, Iss. 1 — Jan. 1, 2001
  • pp: 49–54

Coherent use of opposing lenses for axial resolution increase. II. Power and limitation of nonlinear image restoration

Matthias Nagorni and Stefan W. Hell  »View Author Affiliations


JOSA A, Vol. 18, Issue 1, pp. 49-54 (2001)
http://dx.doi.org/10.1364/JOSAA.18.000049


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Abstract

We analyze the ability of nonlinear image restoration to remove interference artifacts in microscopes that enlarge the axial optical bandwidth through coherent counterpropagating waves. We calculate the images of an elaborate test object as produced by confocal, standing-wave, incoherent illumination interference image interference, and 4Pi confocal microscopes, and we subsequently investigate the extent to which the initial object can be restored by the information allowed by their optical transfer function. We find that nonlinear restoration is successful only if the transfer function is sufficiently contiguous and has amplitudes well above the noise level, as is mostly the case in a two-photon excitation 4Pi confocal microscope.

© 2001 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(100.3020) Image processing : Image reconstruction-restoration
(110.4850) Imaging systems : Optical transfer functions
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: January 28, 2000
Revised Manuscript: August 7, 2000
Manuscript Accepted: August 7, 2000
Published: January 1, 2001

Citation
Matthias Nagorni and Stefan W. Hell, "Coherent use of opposing lenses for axial resolution increase. II. Power and limitation of nonlinear image restoration," J. Opt. Soc. Am. A 18, 49-54 (2001)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-18-1-49

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