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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 19, Iss. 8 — Aug. 1, 2002
  • pp: 1532–1536

Axial apodization in 4Pi-confocal microscopy by annular binary filters

Manuel Martı́nez-Corral, Amparo Pons, and Marı́a-Teresa Caballero  »View Author Affiliations

JOSA A, Vol. 19, Issue 8, pp. 1532-1536 (2002)

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We present a novel technique for considerably decreasing the sidelobe height of the axial point-spread function of one-photon 4Pi-confocal microscopes. By means of a numerical example, in which the ratio between the excitation and the fluorescence wavelengths was set to ϵ = λ exc / λ det = 0.8 , we show that simply inserting a pair of properly designed two-ring binary masks in the illumination set allows the height of the axial sidelobes to be reduced from 20% to 5% of the height of the central peak. This allows one to receive the full benefit of the strong narrowness of the central lobe provided by the 4Pi-confocal technique.

© 2002 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.1790) Microscopy : Confocal microscopy
(220.1230) Optical design and fabrication : Apodization

Original Manuscript: September 18, 2001
Revised Manuscript: March 8, 2002
Manuscript Accepted: March 12, 2002
Published: August 1, 2002

Manuel Martı́nez-Corral, Amparo Pons, and Marı́a-Teresa Caballero, "Axial apodization in 4Pi-confocal microscopy by annular binary filters," J. Opt. Soc. Am. A 19, 1532-1536 (2002)

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