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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 19, Iss. 9 — Sep. 1, 2002
  • pp: 1910–1918

Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy

Jim Swoger, Manuel Martı́nez-Corral, Jan Huisken, and Ernst H. K. Stelzer  »View Author Affiliations

JOSA A, Vol. 19, Issue 9, pp. 1910-1918 (2002)

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The applicability of optical scanning holography (OSH) to the field of microscopic imaging for biological applications is assessed. A generalized mathematical description of OSH that takes into account polarization effects, high numerical apertures, and generalized illumination wave fronts is presented. This description is used to show that the proposed single-beam scanning technique relaxes the restrictions under which OSH functions correctly compared with the conventional double-beam scanning method. It is also shown that, although in general OSH is restricted to thin samples, this condition can be relaxed in nonrefracting fluorescence samples, which are of importance in biological microscopy.

© 2002 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Original Manuscript: December 3, 2001
Revised Manuscript: April 11, 2002
Manuscript Accepted: April 16, 2002
Published: September 1, 2002

Jim Swoger, Manuel Martı́nez-Corral, Jan Huisken, and Ernst H. K. Stelzer, "Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy," J. Opt. Soc. Am. A 19, 1910-1918 (2002)

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