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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 20, Iss. 3 — Mar. 1, 2003
  • pp: 588–595

Soft x-ray holographic computerized tomography imaging: experimental research

Yuxuan Zhang, Shiping Jiang, and Xinyi Zhang  »View Author Affiliations

JOSA A, Vol. 20, Issue 3, pp. 588-595 (2003)

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A high-resolution three-dimensional (3D) imaging technology has been developed, which is a combination of x-ray holography and computerized tomography (CT) technology called holographic computerized tomography (HCT). The theory and experimental techniques on biological specimens with the use of synchrotron radiation are discussed. Projections at different angles are reconstructed with the numerical method of in-line holography, and then the reconstructed data with a higher lateral resolution are used to restore the 3D image by the CT technique. With this method, the degradation caused by the diffraction of x rays is canceled, and 3D images with high resolution of micrometer magnitude in both the lateral and the longitudinal directions are obtained.

© 2003 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(100.6950) Image processing : Tomographic image processing
(340.6720) X-ray optics : Synchrotron radiation
(340.7440) X-ray optics : X-ray imaging

Original Manuscript: June 9, 2002
Revised Manuscript: October 11, 2002
Manuscript Accepted: October 15, 2002
Published: March 1, 2003

Yuxuan Zhang, Shiping Jiang, and Xinyi Zhang, "Soft x-ray holographic computerized tomography imaging: experimental research," J. Opt. Soc. Am. A 20, 588-595 (2003)

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  1. D. Sayer, J. Kirz, R. Feder, D. M. Kim, E. Spiller, “Potential operating region for ultrasoft x-ray microscopy of biological materials,” Science 196, 1339–1340 (1977). [CrossRef]
  2. C. Jacobsen, “Soft x-ray microscopy,” Trends Cell Biol. 9, 44–47 (1999). [CrossRef] [PubMed]
  3. P. C. Cheng, R. Feder, D. M. Shinozaki, K. H. Tan, R. W. Eason, A. Michette, R. J. Rosser, “Soft x-ray contact microscopy,” Nucl. Instrum. Methods Phys. Res. A 246, 668–674 (1986). [CrossRef]
  4. H. Rarback, D. Shu, S. C. Feng, H. Ade, J. Kirz, I. McNulty, D. P. Kern, T. H. P. Chang, Y. Vladimirsky, N. Iskander, D. Attwood, K. McQuaid, S. Rothman, “Scanning x-ray microscope with 75-nm resolution,” Rev. Sci. Instrum. 59, 52–59 (1988). [CrossRef]
  5. G. Schneider, B. Niemann, P. Guttmann, D. Rudolph, G. Schmahl, “Cryo x-ray microscopy,” Synchrotron Radiat. News, March1995, pp. 19–28.
  6. M. R. Howells, C. J. Jacobsen, “X-ray holography,” Synchrotron Radiat. NewsMarch1990, pp. 23–28.
  7. W. S. Haddad, I. McNulty, J. E. Trebes, E. H. Anderson, R. A. Levesque, L. Yang, “Ultrahigh-resolution x-ray tomography,” Science 266, 1213–1215 (1994). [CrossRef] [PubMed]
  8. Y. Wang, C. Jacobsen, J. Maser, A. Osanna, “Soft x-ray microscopy with a cryo scanning transmission x-ray microscope: II. Tomography,” J. Microsc. (Oxford) 197, 80–93 (2000). [CrossRef]
  9. D. Weiss, G. Schneider, B. Niemann, P. Guttmann, D. Rudolph, G. Schmahl, “Computed tomography of cryogenic biological specimens based on x-ray microscopic images,” Ultramicroscopy 84, 185–197 (2000). [CrossRef] [PubMed]
  10. A. K. Jain, ed., Fundamentals of Digital Image Processing (Prentice-Hall, Englewood Cliffs, N. J., 1989), Chap. 10, pp. 431–475.
  11. I. McNulty, J. Trebes, J. Brase, T. J. Yorkey, R. Levesque, H. Szoke, E. H. Anderson, C. Jacobsen, D. Kern, “Experimental demonstration of high resolution three-dimensional x-ray holography,” in Soft X-ray Microscopy, J. Jacobsen, J. E. Trebes, eds., Proc. SPIE1741, 78–84 (1993). [CrossRef]
  12. I. McNulty, “The future of x-ray holography,” Nucl. Instrum. Methods Phys. Res. A 347, 170–176 (1994). [CrossRef]
  13. N. Watanabe, S. Aoki, “Three-dimensional tomography using a soft x-ray holographic microscope and CCD camera,” J. Synchrotron Radiat. 5, 1088–1089 (1998). [CrossRef]
  14. P. Spanne, C. Raven, I. Snigireva, A. Snigirev, “In-line holography and phase-contrast microtomography with high energy x-rays,” Phys. Med. Biol. 44, 741–749 (1999). [CrossRef] [PubMed]
  15. C. Jacobsen, M. R. Howells, J. Kirz, S. Rothman, “X-ray holographic microscopy using photoresists,” J. Opt. Soc. Am. A 7, 1847–1861 (1990). [CrossRef]
  16. A. V. Baez, “A study in diffraction microscopy with special reference to x-rays,” J. Opt. Soc. Am. 42, 756–762 (1952). [CrossRef]
  17. Y. X. Zhang, “Study on x-ray 3D micro-imaging technology using synchrotron radiation,” Ph.D. thesis (University of Science and Technology of China, Hefei, Anhui, China, 2000), Chap. 4.
  18. J. R. Fienup, “Phase retrieval algorithms: a comparison,” Appl. Opt. 21, 2758–2769 (1982). [CrossRef] [PubMed]
  19. L. Onural, P. D. Scott, “Digital decoding of in-line holograms,” Opt. Eng. 26, 1124–1132 (1987). [CrossRef]
  20. R. Gordon, “A tutorial on ART,” IEEE Trans. Nucl. Sci. NS-21, 78–93 (1974). [CrossRef]
  21. T. F. Budinger, G. T. Gullberg, “Three-dimensional reconstruction in nuclear medicine emission imaging,” IEEE Trans. Nucl. Sci. NS-21, 2–20 (1974). [CrossRef]
  22. B. Niemann, D. Rudolph, G. Schmahl, “Soft x-ray imaging zone plates with large zone numbers for microscopic and spectroscopicapplications,” Opt. Commun. 12, 160–163 (1974). [CrossRef]
  23. Y. G. Su, Y. L. Hong, S. J. Fu, X. M. Tao, Y. W. Zhang, “Design and fabrication of condenser zone plates for a soft x-ray microscopy beamline in Hefei,” Rev. Sci. Instrum. 60, 2041–2043 (1989). [CrossRef]
  24. Y. X. Zhang, S. P. Jiang, X. Y. Zhang, “Calibration of the soft x-ray linear monochromator,” J. Univ. Sci. Technol. China 30 (Suppl.), 186–188 (2000).

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