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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 21, Iss. 6 — Jun. 1, 2004
  • pp: 1035–1049

Fluorescence optical diffusion tomography using multiple-frequency data

Adam B. Milstein, Jonathan J. Stott, Seungseok Oh, David A. Boas, R. P. Millane, Charles A. Bouman, and Kevin J. Webb  »View Author Affiliations

JOSA A, Vol. 21, Issue 6, pp. 1035-1049 (2004)

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A method is presented for fluorescence optical diffusion tomography in turbid media using multiple-frequency data. The method uses a frequency-domain diffusion equation model to reconstruct the fluorescent yield and lifetime by means of a Bayesian framework and an efficient, nonlinear optimizer. The method is demonstrated by using simulations and laboratory experiments to show that reconstruction quality can be improved in certain problems through the use of more than one frequency. A broadly applicable mutual information performance metric is also presented and used to investigate the advantages of using multiple modulation frequencies compared with using only one.

© 2004 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.3190) Image processing : Inverse problems
(100.6950) Image processing : Tomographic image processing
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(290.3200) Scattering : Inverse scattering
(290.7050) Scattering : Turbid media

Original Manuscript: September 2, 2003
Revised Manuscript: December 11, 2003
Manuscript Accepted: December 11, 2003
Published: June 1, 2004

Adam B. Milstein, Jonathan J. Stott, Seungseok Oh, David A. Boas, R. P. Millane, Charles A. Bouman, and Kevin J. Webb, "Fluorescence optical diffusion tomography using multiple-frequency data," J. Opt. Soc. Am. A 21, 1035-1049 (2004)

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