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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 23, Iss. 7 — Jul. 1, 2006
  • pp: 1699–1707

Scanning holographic microscopy of three-dimensional fluorescent specimens

Guy Indebetouw and Wenwei Zhong  »View Author Affiliations


JOSA A, Vol. 23, Issue 7, pp. 1699-1707 (2006)
http://dx.doi.org/10.1364/JOSAA.23.001699


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Abstract

We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 μ m of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Holography

History
Original Manuscript: September 16, 2005
Revised Manuscript: January 3, 2006
Manuscript Accepted: January 6, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Guy Indebetouw and Wenwei Zhong, "Scanning holographic microscopy of three-dimensional fluorescent specimens," J. Opt. Soc. Am. A 23, 1699-1707 (2006)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-23-7-1699


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References

  1. J. W. Goodman and R. W. Lawrence, 'Digital image information from electronically detected holograms,' Appl. Phys. Lett. 11, 77-79 (1967). [CrossRef]
  2. D. Gabor, 'A new microscopic principle,' Nature 161, 777-778 (1948). [CrossRef]
  3. E. N. Leith and J. Upatnieks, 'Reconstructed wavefronts and communication theory,' J. Opt. Soc. Am. 52, 1123-1130 (1962). [CrossRef]
  4. U. Schnars and W. P. O. Juptner, 'Digital recording and numerical reconstruction of holograms,' Meas. Sci. Technol. 13, R85-R101 (2002). [CrossRef]
  5. E. Cuche, P. Poscio, and C. Depeursinge, 'Optical tomography at the microscopic scale by means of a numerical low-coherence holographic technique,' in Optical and Imaging Techniques for Biomonitoring II, H. J. Foth, R. Marchesini, and H. Podbielska, eds., Proc. SPIE 2927, 61-66 (1996).
  6. W. Xu, M. H. Jerico, I. A. Meinertzhagen, and H. J. Kreuzer, 'Digital in-line holography for biological applications,' Proc. Natl. Acad. Sci. USA 98, 11301-11305 (2002). [CrossRef]
  7. E. Cuche, F. Bevilacqua, and C. Depeursinge, 'Digital holography for quantitative phase-contrast imaging,' Opt. Lett. 24, 291-293 (1999). [CrossRef]
  8. A. W. Lohmann and W. T. Rhodes, 'Two-pupil synthesis of optical transfer functions,' Appl. Opt. 17, 1141-1151 (1978). [CrossRef] [PubMed]
  9. T.-C. Poon and A. Korpel, 'Optical transfer function of an acousto-optic heterodyning image processor,' Opt. Lett. 4, 317-319 (1979). [CrossRef] [PubMed]
  10. T.-C. Poon, 'Scanning holography and two-dimensional image processing by acousto-optic two-pupil synthesis,' J. Opt. Soc. Am. A 2, 521-527 (1985). [CrossRef]
  11. G. Indebetouw, P. Klysubun, T. Kim, and T.-C. Poon, 'Imaging properties of scanning holographic microscopy,' J. Opt. Soc. Am. A 17, 380-390 (2000). [CrossRef]
  12. B. Schilling, T.-C. Poon, G. Indebetouw, B. Storie, K. Shinoda, and M. Wu, 'Three-dimensional holographic fluorescence microscopy,' Opt. Lett. 22, 1506-1508 (1997). [CrossRef]
  13. G. Indebetouw, 'Properties of a scanning holographic microscope: improved resolution, extended depth of focus, and/or optical sectioning,' J. Mod. Opt. 49, 1479-1500 (2002). [CrossRef]
  14. G. Indebetouw, W. Zhong, and D. Chamberlin-Long, 'Point-spread function synthesis in scanning holographic microscopy,' J. Opt. Soc. Am. A 23, 1708-1717 (2006). [CrossRef]
  15. G. Indebetouw, A. El Maghnouji, and R. Foster, 'Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus,' J. Opt. Soc. Am. A 22, 829-898 (2005). [CrossRef]
  16. A.Diaspro, ed., Confocal and Two-Photon Microscopy: Foundation, Applications, and Advances (Wiley, 2002).
  17. P. Shaw, 'Comparison of wide-field deconvolution and confocal microscopy for 3D imaging,' in Handbook of Biological Confocal Microscopy, 2nd ed., J.B.Pawley, ed. (Plenum, 1995), pp. 373-387.
  18. P. J. Verveer, M. J. Gemkow, and T. M. Jovin, 'A comparison of image restoration approaches applied to three-dimensional confocal and wide-field fluorescence microscopy,' J. Microsc. 183, 50-61 (1999).
  19. C. W. McCutchen, 'Generalized aperture and the three-dimensional diffraction image,' J. Opt. Soc. Am. 54, 240-244 (1964). [CrossRef]
  20. B. E. A. Saleh and M. C. Teich, Fundamentals of Photonics (Wiley, 1991). [CrossRef]

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