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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Editor: Stephen A. Burns
  • Vol. 24, Iss. 12 — Dec. 1, 2007
  • pp: 3728–3734

Quantitative temporal speckle contrast imaging for tissue mechanics

Sean J. Kirkpatrick, Donald D. Duncan, Ruikang K. Wang, and Monica T. Hinds  »View Author Affiliations

JOSA A, Vol. 24, Issue 12, pp. 3728-3734 (2007)

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We demonstrate through a series of simulations that by parameterizing the temporal speckle contrast statistic from a sequence of translating speckle images on a number of experimental constants, the local temporal contrast can be used to quantitatively assess local motion, provided that the spatial and temporal Nyquist sampling criteria are both met. We develop a simple exponential model for quantifying speckle motion for speckle patterns that display arbitrary intensity statistics and provide suggestions for optimizing both the experimental acquisition of speckle data and the temporal contrast analysis of the data. The confounding effects of uncorrelated noise are also discussed. The model is demonstrated by applying it to an optical coherence tomography image sequence of an engineered tissue construct undergoing dynamic compression. Applications to tissue mechanics are shown, although the discussion is equally relevant for fluid motion studies.

© 2007 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(110.4500) Imaging systems : Optical coherence tomography
(110.6150) Imaging systems : Speckle imaging
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.4290) Instrumentation, measurement, and metrology : Nondestructive testing
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging

ToC Category:
Coherence and Statistical Optics

Original Manuscript: June 7, 2007
Revised Manuscript: September 6, 2007
Manuscript Accepted: October 4, 2007
Published: November 20, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Sean J. Kirkpatrick, Donald D. Duncan, Ruikang K. Wang, and Monica T. Hinds, "Quantitative temporal speckle contrast imaging for tissue mechanics," J. Opt. Soc. Am. A 24, 3728-3734 (2007)

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