Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy
JOSA A, Vol. 25, Issue 2, pp. 452-462 (2008)
http://dx.doi.org/10.1364/JOSAA.25.000452
Enhanced HTML 
Acrobat PDF (856 KB)
Abstract
We investigate the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy, using both theoretical and Monte Carlo methods. Our analysis differs from previous work in that it incorporates the data fitting process used in real experiments, allows for the arbitrary choice of excitation and gain waveforms, and calculates lifetimes as well as associated F-values from higher harmonics in the data. Using our analysis, we found different photon efficiencies to those previously reported and were able to propose optimal excitation and gain waveforms. Additionally, we suggest measurement protocols that lead to further improvement in photon efficiency. We compare our results to other techniques for lifetime imaging and consider the implications of our higher-harmonic analysis for multi-exponential lifetime determination.
© 2008 Optical Society of America
OCIS Codes
(000.5490) General : Probability theory, stochastic processes, and statistics
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy
ToC Category:
Fourier Optics and Signal Processing
History
Original Manuscript: July 6, 2007
Revised Manuscript: November 18, 2007
Manuscript Accepted: December 11, 2007
Published: January 29, 2008
Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Alan Elder, Simon Schlachter, and Clemens F. Kaminski, "Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy," J. Opt. Soc. Am. A 25, 452-462 (2008)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-25-2-452
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Fluorescence lifetime imaging with picosecond resolution for biomedical applications (OL)
- Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging (JOSAA)
- On-chip, time-correlated, fluorescence lifetime extraction algorithms and error analysis (JOSAA)
- Statistical analysis and optimization of frequency-domain fluorescence lifetime imaging microscopy using homodyne lock-in detection (JOSAA)
- Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector (OL)
Related Conference Papers
- From extensional to intensional description
- From extensional to intensional description
- Quantum State Transformations, Majorization and Distinguishability
- Quantum State Transformations, Majorization and Distinguishability
- Fluorescence Lifetime Imaging Using Cheap LEDs as Illumination: Application in Microchannels
- Intracellular oxygen sensing using a tunable UV-visible-NIR fluorescence lifetime imaging microscopy system
- Fluorescence lifetime imaging for biomedicine
« Previous Article | Next Article »
OSA is a member of 