OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 25, Iss. 2 — Feb. 1, 2008
  • pp: 452–462

Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy

Alan Elder, Simon Schlachter, and Clemens F. Kaminski  »View Author Affiliations


JOSA A, Vol. 25, Issue 2, pp. 452-462 (2008)
http://dx.doi.org/10.1364/JOSAA.25.000452


View Full Text Article

Enhanced HTML    Acrobat PDF (856 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We investigate the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy, using both theoretical and Monte Carlo methods. Our analysis differs from previous work in that it incorporates the data fitting process used in real experiments, allows for the arbitrary choice of excitation and gain waveforms, and calculates lifetimes as well as associated F-values from higher harmonics in the data. Using our analysis, we found different photon efficiencies to those previously reported and were able to propose optimal excitation and gain waveforms. Additionally, we suggest measurement protocols that lead to further improvement in photon efficiency. We compare our results to other techniques for lifetime imaging and consider the implications of our higher-harmonic analysis for multi-exponential lifetime determination.

© 2008 Optical Society of America

OCIS Codes
(000.5490) General : Probability theory, stochastic processes, and statistics
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Fourier Optics and Signal Processing

History
Original Manuscript: July 6, 2007
Revised Manuscript: November 18, 2007
Manuscript Accepted: December 11, 2007
Published: January 29, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Alan Elder, Simon Schlachter, and Clemens F. Kaminski, "Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy," J. Opt. Soc. Am. A 25, 452-462 (2008)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-25-2-452


Sort:  Year  |  Journal  |  Reset  

References

  1. J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, K. W. Berndt, and M. Johnson, "Fluorescence lifetime imaging," Anal. Biochem. 202, 316-330 (1992). [CrossRef] [PubMed]
  2. K. Suhling, "Fluorescence lifetime imgaging," in Methods Express, Cell Imaging, D.Stephens, ed. (Scion, 2006), pp. 219-245.
  3. W. Becker, A. Bergmann, M. A. Hink, K. König, K. Benndorf, and C. Biskup, "Fluorescence lifetime imaging by time-correlated single-photon counting," Microsc. Res. Tech. 63, 58-66 (2004). [CrossRef]
  4. W. Becker, H. Hickl, C. Zander, K. H. Drexhage, M. Sauer, S. Siebert, and J. Wolfrum, "Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC)," Rev. Sci. Instrum. 70, 1835-1841 (1999). [CrossRef]
  5. K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, "2-D fluorescence lifetime imaging using a time-gated image intensifier," Opt. Commun. 135, 27-31 (1997). [CrossRef]
  6. A. D. Elder, J. H. Frank, J. Swartling, X. Dai, and C. F. Kaminski, "Calibration of a wide-field frequency-domain fluorescence lifetime microscopy system using light emitting diodes as light sources," J. Microsc. 224, 166-180 (2006). [CrossRef]
  7. Q. S. Hanley, V. Subramaniam, D. J. Arndt-Jovin, and T. M. Jovin, "Fluorescence lifetime imaging: multi-point calibration, minimum resolvable differences, and artifact suppression," Cytometry 43, 248-260 (2001). [CrossRef] [PubMed]
  8. A. Draaijer, R. Sanders, and H. C. Gerritsen, "Fluorescence lifetime imaging, a new tool in confocal microscopy," in Handbook of Biological Confocal Microscopy, J.Pawley, ed. (Plenum, 1995), pp. 491-505.
  9. C. J. de Grauw and H. C. Gerritsen, "Multiple time-gate module for fluorescence lifetime imaging," Appl. Spectrosc. 55, 670-678 (2001). [CrossRef]
  10. R. M. Ballew and J. N. Demas, "An error analysis of the rapid lifetime determination method for the evaluation of single exponential decays," Anal. Chem. 61, 30-33 (1989). [CrossRef]
  11. M. Kölner and J. Wolfrum, "How many photons are necessary for fluorescence lifetime measurements?" Chem. Phys. Lett. 200, 199-204 (1992). [CrossRef]
  12. J. Philip and K. Carlsson, "Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging," J. Opt. Soc. Am. 20, 368-379 (2003). [CrossRef]
  13. K. Carlsson and J. Philip, "Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques," Proc. SPIE 4622, 70-78 (2002). [CrossRef]
  14. A. Esposito, T. Oggierc, H. C. Gerritsen, F. Lustenberger, and F. S. Wouters, "All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing," Opt. Express 13, 9812-9821 (2005). [CrossRef] [PubMed]
  15. P. C. Schneider, and R. M. Clegg, "Rapid acquisition, analysis, and display of fuorescence lifetime-resolved images for real-time applications," Rev. Sci. Instrum. 68, 4107-4119 (1997). [CrossRef]
  16. F. R. Boddeke, "Quantitative fluorescence microscopy," Ph.D. dissertation (Technische Universiteit Delft, 1998).
  17. K. Beers, Numerical Methods for Chemical Engineering: Applications in MAT-LAB (Cambridge U. Press, 2006).
  18. A. Squire, P. J. Verveer, and P. I. H. Bastiaens, "Multiple frequency fluorescence lifetime imaging microscopy," J. Microsc. 197, 136-149 (2000). [CrossRef] [PubMed]
  19. A. D. Elder, S. M. Matthews, J. Swartling, K. Yunus, J. H. Frank, C. M. Brennan, A. C. Fisher, and C. F. Kaminski, "Application of frequency-domain fluorescence lifetime imaging microscopy as a quantitative analytical tool for microfluidic devices," Opt. Express 14, 5456-5467 (2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited