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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Stephen A. Burns
  • Vol. 26, Iss. 7 — Jul. 1, 2009
  • pp: 1622–1629

Stochastic transfer function: application to fluorescence microscopy

Ken Hsu, Michael G. Somekh, and Mark C. Pitter  »View Author Affiliations


JOSA A, Vol. 26, Issue 7, pp. 1622-1629 (2009)
http://dx.doi.org/10.1364/JOSAA.26.001622


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Abstract

We develop the concept of the stochastic transfer function (STF) and its application to high-resolution fluorescence microscopy. The STF is directly related to the conventional optical transfer functions but incorporates a probability density function at each spatial frequency. The mean of the STF yields the conventional transfer function; the variance of the STF gives a measure of the noise associated at each spatial frequency. The STF thus provides a more complete picture of the microscope performance in comparison with conventional transfer functions. Here we present the STF for a wide-field fluorescent microscope using both Monte Carlo simulation and probability theory.

© 2009 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 10, 2009
Manuscript Accepted: April 25, 2009
Published: June 19, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ken Hsu, Michael G. Somekh, and Mark C. Pitter, "Stochastic transfer function: application to fluorescence microscopy," J. Opt. Soc. Am. A 26, 1622-1629 (2009)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-26-7-1622

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