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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Editor: Franco Gori
  • Vol. 28, Iss. 10 — Oct. 1, 2011
  • pp: 2026–2032

Precision and accuracy of the analog mean-delay method for high-speed fluorescence lifetime measurement

Young Jae Won, Won-Taek Han, and Dug Young Kim  »View Author Affiliations


JOSA A, Vol. 28, Issue 10, pp. 2026-2032 (2011)
http://dx.doi.org/10.1364/JOSAA.28.002026


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Abstract

The analog mean-delay (AMD) method is a new alternative method to measure the lifetime of a fluorescence molecule. Because of its powerful advantages of accurate lifetime determination, good photon economy, and a high photon detection rate, the AMD method is considered to be very suitable for high-speed confocal fluorescence lifetime imaging microscopy (FLIM). For the practical usage of the AMD method in FLIM (AMD-FLIM), detailed study on various experimental conditions and parameters that affect the precision and the accuracy of the AMD method is required. In this paper, we present the relation between the precision and accuracy of the lifetime versus iteration number in the AMD method, the best cutoff frequency of a low-pass filter used in the AMD-FLIM system for a given fluorophore, and the optimum position and width of the integration window by using Monte Carlo simulations and a series of AMD-FLIM experiments.

© 2011 Optical Society of America

OCIS Codes
(000.2170) General : Equipment and techniques
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 11, 2011
Manuscript Accepted: August 16, 2011
Published: September 9, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Young Jae Won, Won-Taek Han, and Dug Young Kim, "Precision and accuracy of the analog mean-delay method for high-speed fluorescence lifetime measurement," J. Opt. Soc. Am. A 28, 2026-2032 (2011)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-28-10-2026


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