OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 11, Iss. 5 — May. 1, 1994
  • pp: 1619–1627

Three-dimensional transfer functions in 4Pi confocal microscopes

Min Gu and C. J. R. Sheppard  »View Author Affiliations


JOSA A, Vol. 11, Issue 5, pp. 1619-1627 (1994)
http://dx.doi.org/10.1364/JOSAA.11.001619


View Full Text Article

Enhanced HTML    Acrobat PDF (983 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We investigate three-dimensional transfer functions in order to understand image formation in the recently proposed 4Pi confocal microscope [ J. Opt. Soc. Am. A 9, 2159 ( 1992); Opt. Commun. 93, 277 ( 1992)]. Both nonfluorescence and fluorescence imaging methods are considered, and the effects of apodization functions are taken into account. It is shown that for a 4Pi confocal system of finite-sized aperture the cutoff spatial frequency in the axial direction is larger than that in the transverse direction; this behavior is opposite that of conventional confocal systems, explaining the observation of high axial resolution in 4Pi confocal systems. In addition, the three-dimensional optical transfer function for a conventional confocal fluorescence microscope of high aperture is presented.

© 1994 Optical Society of America

History
Original Manuscript: June 18, 1993
Revised Manuscript: November 29, 1993
Manuscript Accepted: November 30, 1993
Published: May 1, 1994

Citation
Min Gu and C. J. R. Sheppard, "Three-dimensional transfer functions in 4Pi confocal microscopes," J. Opt. Soc. Am. A 11, 1619-1627 (1994)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-11-5-1619

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited