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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 15, Iss. 8 — Aug. 1, 1998
  • pp: 2052–2058

Image formation in turbid media under a microscope

X. S. Gan, S. P. Schilders, and Min Gu  »View Author Affiliations


JOSA A, Vol. 15, Issue 8, pp. 2052-2058 (1998)
http://dx.doi.org/10.1364/JOSAA.15.002052


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Abstract

Image formation in a turbid medium under a microscope is studied both theoretically and experimentally. In particular, the relationship of image resolution to scattered photons, which experience different numbers of scattering events, is explored in a scanning microscope. The effects of the numerical aperture of a microscope objective and the detector size on image resolution that is contributed by scattered photons are carefully investigated. The results show that for an object embedded in a turbid medium of a thickness of 12 scattering mean-free-path lengths, transverse resolution of an image contributed by scattered photons is much lower than the diffraction-limited resolution. A criterion for determining the efficiency of a gating method is proposed in terms of the relationship of resolution to signal strength.

© 1998 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.7050) Imaging systems : Turbid media

History
Original Manuscript: January 9, 1998
Revised Manuscript: March 24, 1998
Manuscript Accepted: April 23, 1998
Published: August 1, 1998

Citation
X. S. Gan, S. P. Schilders, and Min Gu, "Image formation in turbid media under a microscope," J. Opt. Soc. Am. A 15, 2052-2058 (1998)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-15-8-2052

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