OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 15, Iss. 9 — Sep. 1, 1998
  • pp: 2275–2287

Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions

Ibrahim Akduman, Ulrich Brand, Jan Grochmalicki, Gerard Hester, Roy Pike, and Mario Bertero  »View Author Affiliations


JOSA A, Vol. 15, Issue 9, pp. 2275-2287 (1998)
http://dx.doi.org/10.1364/JOSAA.15.002275


View Full Text Article

Enhanced HTML    Acrobat PDF (443 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A singular-value-decomposition analysis of the imaging kernel for three-dimensional fluorescent laser scanning microscopy at a high numerical aperture (NA) is presented. The design and superresolving performance of image-plane binary optical masks are then derived, and new computational techniques for calculating these masks are given. Initial experimental results with a microscope equipped with such a mask at NA=1.3 are presented. The improvement in both contrast and resolution over the confocal and type 1 instruments is demonstrated.

© 1998 Optical Society of America

OCIS Codes
(070.4560) Fourier optics and signal processing : Data processing by optical means
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: January 27, 1998
Manuscript Accepted: May 4, 1998
Published: September 1, 1998

Citation
Ibrahim Akduman, Ulrich Brand, Jan Grochmalicki, Gerard Hester, Roy Pike, and Mario Bertero, "Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions," J. Opt. Soc. Am. A 15, 2275-2287 (1998)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-15-9-2275


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. G. Walker, E. R. Pike, R. E. Davies, M. R. Young, G. J. Brakenhoff, M. Bertero, “Superresolving scanning optical microscopy using holographic optical processing,” J. Opt. Soc. Am. A 10, 59–64 (1993). [CrossRef]
  2. M. Bertero, P. Boccacci, R. E. Davies, F. Malfanti, E. R. Pike, J. G. Walker, “Superresolution in confocal scanning microscopy: IV. Theory of data inversion by the use of optical masks,” Inverse Probl. 8, 1–23 (1992). [CrossRef]
  3. M. R. Young, S. H. Jiang, R. E. Davies, J. G. Walker, E. R. Pike, M. Bertero, “Experimental confirmation of superresolution in coherent confocal scanning microscopy using optical masks,” J. Microsc. 165, 131–138 (1992). [CrossRef]
  4. J. Grochmalicki, E. R. Pike, J. G. Walker, “Experimental confirmation of superresolution in incoherent scanning microscopy,” Pure Appl. Opt. 2, 565–568 (1993). [CrossRef]
  5. M. Bertero, P. Boccacci, G. J. Brakenhoff, F. Malfanti, H. T. M. van der Voort, “Three-dimensional image restoration and superresolution in fluorescence confocal microscopy,” J. Microsc. 157, 3–20 (1990). [CrossRef]
  6. M. Bertero, P. Boccacci, F. Malfanti, E. R. Pike, “Superresolution in confocal scanning microscopy: V. Axial superresolution in the incoherent case,” Inverse Probl. 10, 1059–1077 (1994). [CrossRef]
  7. V. S. Ignatowsky, “Diffraction by an objective lens with arbitrary aperture,” Trans. Opt. Inst. Petrograd 1(4), 1–36 (1921) (in Russian).
  8. B. Richards, E. Wolf, “Electromagnetic diffraction in op-tical systems: II. Structure of the image field in aplanatic systems,” Proc. R. Soc. London, Ser. A 253, 358–379 (1959). [CrossRef]
  9. M. Bertero, P. Boccacci, M. Defrise, C. De Mol, E. R. Pike, “Superresolution in confocal scanning microscopy: II. The incoherent case,” Inverse Probl. 5, 441–461 (1989). [CrossRef]
  10. M. Bertero, P. Boccacci, “Computation of the singular system for a class of integral operators related to data inversion in confocal microscopy,” Inverse Probl. 5, 935–957 (1989). [CrossRef]
  11. A. Papoulis, “Hankel transforms,” in Systems and Transforms with Applications in Optics (McGraw-Hill, New York, 1968).
  12. H. E. Fettis, “Lommel-type integrals involving three Bessel functions,” J. Math. Phys. (Paris) 36, 88–95 (1957).
  13. J. Grochmalicki, E. R. Pike, J. G. Walker, M. Bertero, P. Boccacci, R. E. Davies, “Superresolving masks for incoherent scanning microscopy,” J. Opt. Soc. Am. A 10, 1074–1077 (1993). [CrossRef]
  14. T. Wilson, C. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, London, 1984).
  15. A. E. Siegman, “Quasi-fast Hankel transform,” Opt. Lett. 1, 13–15 (1977). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited