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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 16, Iss. 12 — Dec. 1, 1999
  • pp: 2959–2967

Light-scattering properties of undiluted human blood subjected to simple shear

Wiendelt Steenbergen, Roy Kolkman, and Frits de Mul  »View Author Affiliations

JOSA A, Vol. 16, Issue 12, pp. 2959-2967 (1999)

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An experimental investigation was performed into the effect of simple shear on the light-scattering properties of undiluted human blood. Undiluted human blood was enclosed between two glass plates with an adjustable separation between 30 and 120 μm and with one plate moving parallel to the other. For various shear rates and layer thicknesses, the angular light distribution and the collimated transmission were measured for 633-nm light. For shear rates above 150 s-1, the transmission results directly yielded a total attenuation coefficient of 120 mm-1. At lower shear rates the total attenuation followed an irregular pattern. From the angular intensity distributions, the anisotropy for single scattering was deduced by inverse Monte Carlo simulations. A continuous increase of the average cosine g with the shear rate was observed, with g in the range 0.95–0.975.

© 1999 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5280) Medical optics and biotechnology : Photon migration
(170.5380) Medical optics and biotechnology : Physiology
(170.7050) Medical optics and biotechnology : Turbid media
(290.2200) Scattering : Extinction
(290.5820) Scattering : Scattering measurements

Original Manuscript: April 20, 1999
Revised Manuscript: August 19, 1999
Manuscript Accepted: August 19, 1999
Published: December 1, 1999

Wiendelt Steenbergen, Roy Kolkman, and Frits de Mul, "Light-scattering properties of undiluted human blood subjected to simple shear," J. Opt. Soc. Am. A 16, 2959-2967 (1999)

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