Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy
JOSA A, Vol. 16, Issue 9, pp. 2185-2199 (1999)
http://dx.doi.org/10.1364/JOSAA.16.002185
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Abstract
Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.
© 1999 Optical Society of America
OCIS Codes
(110.2990) Imaging systems : Image formation theory
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.6900) Microscopy : Three-dimensional microscopy
Citation
Chrysanthe Preza, Donald L. Snyder, and José-Angel Conchello, "Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy," J. Opt. Soc. Am. A 16, 2185-2199 (1999)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-16-9-2185
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