Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.
© 1999 Optical Society of America
Original Manuscript: August 10, 1998
Revised Manuscript: February 8, 1999
Manuscript Accepted: February 8, 1999
Published: September 1, 1999
Chrysanthe Preza, Donald L. Snyder, and José-Angel Conchello, "Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy," J. Opt. Soc. Am. A 16, 2185-2199 (1999)