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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 16, Iss. 9 — Sep. 1, 1999
  • pp: 2185–2199

Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy

Chrysanthe Preza, Donald L. Snyder, and José-Angel Conchello  »View Author Affiliations


JOSA A, Vol. 16, Issue 9, pp. 2185-2199 (1999)
http://dx.doi.org/10.1364/JOSAA.16.002185


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Abstract

Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.

© 1999 Optical Society of America

OCIS Codes
(110.2990) Imaging systems : Image formation theory
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.6900) Microscopy : Three-dimensional microscopy

History
Original Manuscript: August 10, 1998
Revised Manuscript: February 8, 1999
Manuscript Accepted: February 8, 1999
Published: September 1, 1999

Citation
Chrysanthe Preza, Donald L. Snyder, and José-Angel Conchello, "Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy," J. Opt. Soc. Am. A 16, 2185-2199 (1999)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-16-9-2185


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