We analyzed the ratio of L:M cone photopigment mRNA in the retinas of Old World monkeys, using the method of rapid polymerase chain reaction–single-strand conformation polymorphism. The L:M cone pigment mRNA ratio in whole retina ranged from 0.6 to 7.0, with a mean of ∼1.6 (standard deviation, ±0.56; n=26). There was no change in this ratio with eccentricity up to 9 mm (∼45°), though the ratio was ∼30% greater in temporal than in nasal retina. The mRNA ratios are in good agreement with the L:M cone ratio in these same retinas, inferred from electrophysiological recordings of cone signal gain in horizontal cell interneurons. The correlation between mRNA ratios and physiological cone gain ratio supports the conclusion that both measures reflect the relative number of L and M cones.

© 2000 Optical Society of America

[Optical Society of America ]

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• General method for deriving an XYZ tristimulus space exemplified by use of the Stiles–Burch₁₉₅₅ 2° color matching data (JOSAA)
• Physiology of L- and M-cone inputs to H1 horizontal cells in the primate retina (JOSAA)
• Cost of cone coupling to trichromacy in primate fovea (JOSAA)
• Spatial summation in human cone mechanisms from 0° to 20° in the superior retina (JOSAA)
• Method of targeted delivery of laser beam to isolated retinal rods by fiber optics (BOE)

Related Conference Papers

• Incoherent manipulation of the photoactive yellow protein photocycle with dispersed pump-dump-probe spectroscopy
• Traction centered nasal to the optic nerve head in myopic and normal observers: Opto-kinetic nystagmus (OKN) induces transient changes in inferred photoreceptor orientations <i></i>
• Inter-Photoreceptor Distance Calibrated by Axial Length in Adaptive Optics Fundus Camera
• Fast Optical Imaging of Neural Activation in Isolated Retina
• The Stiles-Crawford Effects, 75 Years: A Brief History and Experiences at the National Physical Laboratory (Teddington, UK) with W. S. Stiles and B. H. Crawford)