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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 17, Iss. 3 — Mar. 1, 2000
  • pp: 538–544

Interindividual and topographical variation of L:M cone ratios in monkey retinas

Samir S. Deeb, Lisa C. Diller, David R. Williams, and Dennis M. Dacey  »View Author Affiliations

JOSA A, Vol. 17, Issue 3, pp. 538-544 (2000)

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We analyzed the ratio of L:M cone photopigment mRNA in the retinas of Old World monkeys, using the method of rapid polymerase chain reaction–single-strand conformation polymorphism. The L:M cone pigment mRNA ratio in whole retina ranged from 0.6 to 7.0, with a mean of ∼1.6 (standard deviation, ±0.56; n=26). There was no change in this ratio with eccentricity up to 9 mm (∼45°), though the ratio was ∼30% greater in temporal than in nasal retina. The mRNA ratios are in good agreement with the L:M cone ratio in these same retinas, inferred from electrophysiological recordings of cone signal gain in horizontal cell interneurons. The correlation between mRNA ratios and physiological cone gain ratio supports the conclusion that both measures reflect the relative number of L and M cones.

© 2000 Optical Society of America

OCIS Codes
(330.0330) Vision, color, and visual optics : Vision, color, and visual optics
(330.5310) Vision, color, and visual optics : Vision - photoreceptors
(330.5380) Vision, color, and visual optics : Physiology

Original Manuscript: July 21, 1999
Revised Manuscript: November 1, 1999
Manuscript Accepted: November 3, 1999
Published: March 1, 2000

Samir S. Deeb, Lisa C. Diller, David R. Williams, and Dennis M. Dacey, "Interindividual and topographical variation of L:M cone ratios in monkey retinas," J. Opt. Soc. Am. A 17, 538-544 (2000)

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