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Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

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Abstract

The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.

© 2000 Optical Society of America

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