The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.
© 2000 Optical Society of America
(110.4100) Imaging systems : Modulation transfer function
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(180.6900) Microscopy : Three-dimensional microscopy
(290.4020) Scattering : Mie theory
Ben Ovryn and Steven H. Izen, "Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope," J. Opt. Soc. Am. A 17, 1202-1213 (2000)