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Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 17, Iss. 7 — Jul. 1, 2000
  • pp: 1202–1213

Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

Ben Ovryn and Steven H. Izen  »View Author Affiliations


JOSA A, Vol. 17, Issue 7, pp. 1202-1213 (2000)
http://dx.doi.org/10.1364/JOSAA.17.001202


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Abstract

The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.

© 2000 Optical Society of America

OCIS Codes
(110.4100) Imaging systems : Modulation transfer function
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(180.6900) Microscopy : Three-dimensional microscopy
(290.4020) Scattering : Mie theory

Citation
Ben Ovryn and Steven H. Izen, "Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope," J. Opt. Soc. Am. A 17, 1202-1213 (2000)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-17-7-1202

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