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Journal of the Optical Society of America A

Journal of the Optical Society of America A


  • Vol. 18, Iss. 4 — Apr. 1, 2001
  • pp: 771–776

Three-dimensional transfer functions of coherent anti-Stokes Raman scattering microscopy

Mamoru Hashimoto and Tsutomu Araki  »View Author Affiliations

JOSA A, Vol. 18, Issue 4, pp. 771-776 (2001)

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The three-dimensional coherent transfer function of confocal coherent anti-Stokes Raman scattering microscopy was derived theoretically. The three-dimensional optical transfer function was also derived under the weak-contrast assumption. The effect of a pinhole in front of the detector on the optical transfer function was estimated, and it was found that the cutoff frequency of the optical transfer function is independent of the pinhole. Micrometer-order spatial resolution along the optical axis was also experimentally demonstrated.

© 2001 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(110.6880) Imaging systems : Three-dimensional image acquisition
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

Original Manuscript: January 24, 2000
Revised Manuscript: August 28, 2000
Manuscript Accepted: October 19, 2000
Published: April 1, 2001

Mamoru Hashimoto and Tsutomu Araki, "Three-dimensional transfer functions of coherent anti-Stokes Raman scattering microscopy," J. Opt. Soc. Am. A 18, 771-776 (2001)

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  1. T. Wilson, ed., Confocal Microscopy (Academic, London, 1990).
  2. W. Denk, J. H. Strickler, W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  3. W. Denk, K. R. Delaney, A. Gelperin, D. Kleinfeld, B. W. Strowbridge, D. W. Tank, R. Yuste, “Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy,” J. Neurosci. Methods 54, 151–162 (1994). [CrossRef] [PubMed]
  4. G. J. Puppels, F. F. M. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, T. M. Jovin, “Studying single living cells and chromosomes by confocal Raman micro-spectroscopy,” Nature 347, 301–303 (1990). [CrossRef] [PubMed]
  5. N. M. Sijtsema, S. D. Wouters, C. J. de Grauw, C. Otto, J. Greve, “Confocal direct imaging Raman microscope: design and applications in biology,” Appl. Spectrosc. 52, 348–355 (1998). [CrossRef]
  6. M. D. Duncan, J. Reintjes, T. J. Manuccia, “Scanning coherent anti-Stokes Raman microscope,” Opt. Lett. 7, 350–352 (1982). [CrossRef] [PubMed]
  7. M. D. Duncan, J. Reintjes, T. J. Manuccia, “Imaging biological compounds using the coherent anti-Stokes Raman scattering microscope,” Opt. Eng. 24, 352–355 (1985). [CrossRef]
  8. M. D. Duncan, “Molecular discrimination and contrast enhancement using a scanning coherent anti-Stokes Raman microscope,” Opt. Commun. 50, 307–312 (1984). [CrossRef]
  9. Y. R. Shen, The Principles of Nonlinear Optics (Wiley, New York, 1984).
  10. M. D. Levenson, S. Kano, Introduction to Nonlinear Laser Spectroscopy (Academic, Orlando, Fla., 1988).
  11. M. Hashimoto, T. Araki, “Coherent anti-Stokes Raman scattering microscope,” in 18th Congress of the International Commission for Optics: Optics for the Next Millennium, A. J. Glass, J. W. Goodman, M. Chang, A. H. Guenther, T. Asakura, eds., Proc. SPIE3749, 496–497 (1999). [CrossRef]
  12. A. Zumbusch, G. R. Holtom, X. S. Xie, “Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,” Phys. Rev. Lett. 82, 4142–4145 (1999). [CrossRef]
  13. N. Streibl, “Three-dimensional imaging by a microscope,” J. Opt. Soc. Am. A 2, 121–127 (1985). [CrossRef]
  14. O. Nakamura, S. Kawata, “Three-dimensional transfer-function analysis of the tomographic capability of a confocal fluorescence microscope,” J. Opt. Soc. Am. A 7, 522–526 (1990). [CrossRef] [PubMed]
  15. J. W. Nibler, W. M. Shaub, J. R. McDonald, A. B. Harvey, “Coherent anti-Stokes Raman spectroscopy,” in Vibrational Spectra and Structure, J. R. During, ed. (Elsevier, New York, 1977), Vol. 6, Chap. 3, pp. 173–225.

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