OSA's Digital Library

Journal of the Optical Society of America A

Journal of the Optical Society of America A

| OPTICS, IMAGE SCIENCE, AND VISION

  • Vol. 18, Iss. 6 — Jun. 1, 2001
  • pp: 1212–1230

Macular pigment density measured by autofluorescence spectrometry: comparison with reflectometry and heterochromatic flicker photometry

François C. Delori, Douglas G. Goger, Billy R. Hammond, D. Max Snodderly, and Stephen A. Burns  »View Author Affiliations


JOSA A, Vol. 18, Issue 6, pp. 1212-1230 (2001)
http://dx.doi.org/10.1364/JOSAA.18.001212


View Full Text Article

Enhanced HTML    Acrobat PDF (1160 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a technique for estimating the density of the human macular pigment noninvasively that takes advantage of the autofluorescence of lipofuscin, which is normally present in the human retinal pigment epithelium. By measuring the intensity of fluorescence at 710 nm, where macular pigment has essentially zero absorption, and stimulating the fluorescence with two wavelengths, one well absorbed by macular pigment and the other minimally absorbed by macular pigment, we can make accurate single-pass measurements of the macular pigment density. We used the technique to measure macular pigment density in a group of 159 subjects with normal retinal status ranging in age between 15 and 80 years. Average macular pigment density was 0.48 ± 0.16 density unit (D.U.) for a 2°-diameter test field. We show that these estimates are highly correlated with reflectometric (mean: 0.23±0.07 D.U.) and psychophysical (mean: 0.37±0.26 D.U.; obtained by heterochromatic flicker photometry) estimates of macular pigment in the same subjects, despite the fact that systematic differences in the estimated density exist between techniques. Repeat measurements over both short- and long-time intervals indicate that the autofluorescence technique is reproducible: The mean absolute difference between estimates was less than 0.05 D.U., superior to the reproducibility obtained by reflectometry and flicker photometry. To understand the systematic differences between density estimates obtained from the different methods, we analyzed the underlying assumptions of each technique. Specifically, we looked at the effect of self-screening by visual pigment, the effect of changes in optical property of the deeper retinal layers, including the role of retinal pigmented epithelium melanin, and the role of secondary fluorophores and reflectors in the anterior layers of the retina.

© 2001 Optical Society of America

OCIS Codes
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(300.0300) Spectroscopy : Spectroscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment
(330.5510) Vision, color, and visual optics : Psychophysics

History
Original Manuscript: May 23, 2000
Revised Manuscript: September 14, 2000
Manuscript Accepted: November 8, 2000
Published: June 1, 2001

Citation
François C. Delori, Douglas G. Goger, Billy R. Hammond, D. Max Snodderly, and Stephen A. Burns, "Macular pigment density measured by autofluorescence spectrometry: comparison with reflectometry and heterochromatic flicker photometry," J. Opt. Soc. Am. A 18, 1212-1230 (2001)
http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-18-6-1212

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited